Vascular Endothelial Growth Factor and Basic Fibroblast Growth Factor Levels in the Vitreous of Patients with Proliferative Diabetic Retinopathy

血管內皮生長因子(VEGF)及鹼基纖維母細胞生長因子(bFGF)在增殖性糖尿病視網膜病變病人玻璃體中之濃度

曹中傑(Chung-Chieh Tsao) ； 黃正賢(Jen-Shang Huang) ； 黃振宇(Jen-Yu Huang) ； 陳慕師(Muh-Shy Chen)

《中華民國眼科醫學會雜誌》 41卷2期 (2002/06) Pp. 121-128

https://doi.org/10.30048/ACTASOS.200206.0006

摘要

Purpose: If the release of VEGF or bFGF provides important stimulates of neovascularization in eye disorders the presence of VEGF or bFGF should theoretically be detected in vitreous fluids. To determine the relative levels of vascular endothelial growth factor (VEGF) and basic fibroblast growth factor (bFGF) in the vitreous of patients with proliferative diabetic retinopathy (PDR).
Methods: A sandwich enzyme- linked immunosorbent assay (ELISA) (R&D systems) was used to quantitate levels of VEGF and bFGF in the vitreous of 67 patients undergoing vitrectomy for a variety of retinal conditions, including PDR, branch retinal vein occlusion (BRVO), age- related macular degeneration (ARMD), retinal detachment (RD), retained lens material, epiretinal membrane, macular hole, and vitreous opacity.
Results: VEGF levels of vitreous were significantly higher in eyes with PDR (1724 386 pg/ml) than in eyes without PDR (21 23 pg/ml) (P＜0.01). Furthermore, the vitreous concentration of VEGF were higher in patients with active PDR (2048 pg/ml) than in patients with quiescent PDR (74 pg/ml). Patients with PDR had a mean vitreous bFGF concentration of 10.3 9.2 pg/ml compared with 9.5 8.9 pg/ml in non-diabetic controls. No statistically signify- cant difference were found in the vetreous concentration of bFGF between both groups.
Conclusions: Our study documented increased levels of VEGF in the vitreous specimen from patients with PDR, particularly those with active PDR.

並列摘要

Purpose: If the release of VEGF or bFGF provides important stimulates of neovascularization in eye disorders the presence of VEGF or bFGF should theoretically be detected in vitreous fluids. To determine the relative levels of vascular endothelial growth factor (VEGF) and basic fibroblast growth factor (bFGF) in the vitreous of patients with proliferative diabetic retinopathy (PDR).
Methods: A sandwich enzyme- linked immunosorbent assay (ELISA) (R&D systems) was used to quantitate levels of VEGF and bFGF in the vitreous of 67 patients undergoing vitrectomy for a variety of retinal conditions, including PDR, branch retinal vein occlusion (BRVO), age- related macular degeneration (ARMD), retinal detachment (RD), retained lens material, epiretinal membrane, macular hole, and vitreous opacity.
Results: VEGF levels of vitreous were significantly higher in eyes with PDR (1724 386 pg/ml) than in eyes without PDR (21 23 pg/ml) (P＜0.01). Furthermore, the vitreous concentration of VEGF were higher in patients with active PDR (2048 pg/ml) than in patients with quiescent PDR (74 pg/ml). Patients with PDR had a mean vitreous bFGF concentration of 10.3 9.2 pg/ml compared with 9.5 8.9 pg/ml in non-diabetic controls. No statistically signify- cant difference were found in the vetreous concentration of bFGF between both groups.
Conclusions: Our study documented increased levels of VEGF in the vitreous specimen from patients with PDR, particularly those with active PDR.

並列關鍵字

vascular endothelial growth factor ； basic fibroblast growth factor ； proliferative diabetic retinopathy