- 期刊
Mitochondrial DNA Variation in Natural Populations of Drosophila Immigrans
大果蠅（Drosophila immigrans）自然族群之粒線體DNA之變異
摘要
由越南、臺灣及日本所採集的7個大果蠅（Drosophila immigrans）自然族群的果蠅，利用12種不同的限制酶（Restriction enzymes）今析其粒線體DNA，總共分析了18個粒線體DNA的單倍體型（Haplotypes），每一核苷酸位置之核苷酸置換率（πij）為0.002～0.016，族群內粒線體DNA之分歧度（π）估計為0.0014～0.0077，而族群與族群之間之分歧度（d）則為-0.004～0.0045。由粒線體DNA單倍體型的頻度估計出每一個族群之遺傳分化度（Gst）為0.205。由UPGMA分析這七個族群之核苷酸分歧度顯示出，臺灣和日本南部的族群成為一個群聚（cluster），而日本北部的族群和上面這兩個地方的族群遺傳組成上有些差異，越南的族群其遺傳組成和臺灣和日本的族群差異較大。
關鍵字
種內變異並列摘要
The mitochondrial DNA restriction patterns of seven natural populations of Drosophila immigrans from North Vietnam, Taiwan, and Japan were analyzed. Using twelve restriction endonucleases we recognized a total of 18 mtDNA haplotypes. The mean number of nucleotide substitutions between mtDNA haplotypes (πij) was estimated to be from 0.002 to 0.016. The amount of mtDNA divergence within a population (π) was estimated to be from 0.0014 to 0.0077, while the net nucleotide differentiation among populations (d) ranged from - 0.0004 to 0.0045. The estimated Gst value from mtDNA haplotype frequencies of each population was 0.205, indicating relatively uniform genetic constitution throughout the investigated D. immigrans populations. However, nucleotide diversity of the seven populations showed that the Taiwan and the southern Japan populations formed one cluster; the Sapporo population is slightly distanced from this cluster. The latitudinal systematic mtDNA differentiation among populations is discussed.