將不同時期之中國對蝦蝦苗以靜止換水(Static renewal)方式暴露於一連串亞硝酸一氮溶液中。在所有測試之蝦苗中以無節幼蟲對亞硝酸最敏感,而以後期幼蟲之抵抗力最強。對於N3(無節幼蟲第3期)、Z2(眼幼蟲第2期)、M2(糠蝦蟲第2期)、PL12(後期幼蟲第12期)之24小時LC50分別為23.12、56.34、73.61及67.71毫克/升(鹽度30ppt、pH 8.21 ± 0.03)。對於Z2、M2及PL12之48小時LC50勞別為29.89、25.54及52.14毫克/升。亞硝酸對於PL12之「閾值」在108小時,對於PL12之“incipient LC50”為13.83毫克/升亞硝酸一氮,在繁殖培育中國對蝦蝦苗,亞硝酸-氮之安全基準為1.38毫克/升。
Penaeus chinensis larvae at the nauplius stage are the most susceptible, while postlarvae are the most tolerant to nitrite. The 24 hr LC50s were 23.12, 56.34, 73.61, and 67.71 mg/l nitrite-N for nauplius third substage (N3), zoea second substage (Z2), mysis second substage (M2), and postlarva twelfth substage (PL12), respectively in 30 ppt seawater at pH 8.21 ± 0.03. The ”threshold time” was found to be 108 hr for PL12 on a toxicity curve approaching an asymptote. Based on the ”incipient LC50” and an application factor of 0.1, the ”safe level” for rearing P. chinensis larvae is estimated to be 1.38 mg/l nitrite-N.