Transgenic Fish: Ideal Models for Basic Research and Biotechnological Applications

轉殖基因魚：基礎科學研究及生物科技應用之理想模式

陳鐵雄(Thomas T. Chen) ； 陸振岡(Jenn-Kan Lu) ； Mike J. Shamblott ； 鄭美潔(Clara M. Cheng) ； 林純民(Chun-Mean Lin) ； Jane C. Burns ； Renate Reimschuessel ； Nagaraj Chatakondi ； Rex A. Dunham

《Zoological Studies》 34卷4期 (1995/07) Pp. 215-234

摘要

用人工方法將外來之基因轉殖入生物原有之基因體者稱為轉基因生物。自1985年來，顯微注射法及電破法已廣泛被使用生產各種不同之轉基因魚類。這些轉基因魚類可用為基礎科學研究及生物科技應用之模型。使用近年來本實驗室及其他實驗室所得之研究結果為例，本文將闡釋轉殖基因魚之利用價值。

關鍵字

基因轉殖技術 ； 生長激素 ； 類胰島素生長因子 ； 顯微注射法 ； 電破法

並列摘要

Organisms into which heterologous DNA has been artifically introduced and stably integrated in their genomes are termed transgenic. Since 1985, a wide variety of transgenic fish species have been produced by microinjecting or electroporation into fertilized or unfertilized eggs. These transgenic fish can serve as excellent experimental models for basic scientific investigations as well as biotechnical applications. In this paper, using research results generated in our laboratories and those of others as examples, we will review the current status of the transgenic fish research and illustrate the potential application of this technology in both basic research and biotechnological applications.

並列關鍵字

Gene transfer technology ； Growth hormone ； Insulin-like growth factor ； Microinjection ； Electroporation