Universal Primers for Amplification of the Complete Mitochondrial 12S rRNA Gene in Vertebrates

脊椎動物粒線體tRNA^PHE和16S rRNA之保守區經比對後設計成引子12SR和12SL。這些萬有引子可以廣泛地使用於脊椎動物各個主群的動物種，而能利用聚合酶鏈反應來增幅約1.3 kb的去氧核醣核酸片段。此產物的長度差異在各群脊椎動物間很小。進一步的序列分析顯示此片段含有完整的12S rRNA和tRNA^PHE及約200 kb的16S rRNA。研究分類群後發現上述的引子能用以增幅完整粒線體12S rRNA。在12S rRNA基因被廣泛地用於不同層級類緣分析的同時，這些引子組更能被用在脊椎動物較高階的類緣關係。

關鍵字

12SL和12SR ；引子；聚合酶鏈反應增幅作用；脊椎動物

並列摘要

The conserved regions of tRNAPHE and 16S rRNA in the vertebrate mitochondrial genome were compared in order to design the primers, 12SR and 12SL. These universal primers can be broadly used to amplify a 1.3-kb DNA fragment by polymerase chain reaction (PCR) over a wide range of major vertebrate lineages represented by the species listed in the text. There is little length variation of the PCR product among different taxa. Further sequence analysis revealed that the fragment contains complete lengths of 12S rRNA and tRNAVAL, and that the length of 16S rRNA is 200 bp. In tests through all representative taxa investigated, the above 2 primers could amplify the complete 12S rRNA gene from all representative taxa investigated. As the 12S rRNA gene is widely used for phylogenetic analyses among different hierarchies, the use of these primer sets for study of higher-category phylogenies in vertebrates now becomes possible.

並列關鍵字

12SR and 12SL ； Primers ； PCR amplification ； Vertebrates

國際替代計量

Universal Primers for Amplification of the Complete Mitochondrial 12S rRNA Gene in Vertebrates

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