Cloning and Characterization of cDNA Encoding the Porcine Mcl-1 Protein

豬Mcl-1 cDNA之選殖與組織表現之研究

陳志毅(Jyh-Yih Chen) ； 黃尉東(Wei-Tung Huang) ； 吳金洌(Jen-Leih Wu)

《Zoological Studies》 41卷2期 (2002/04) Pp. 136-143

摘要

從豬之肌肉互補核苷酸基因庫中選殖出Mcl-1互補核苷酸序列，其由2487個核苷酸組成，並可轉譯出152個胺基酸。以豬之Mcl-1胺基酸序列比對大鼠、人及斑馬魚之已發表序列顯示，其相似性分別為83.9%、85.3%及50.0%。另以定量聚合酶連鎖反應儀器分析顯示，Mcl-1於豬之子宮、肺及肝臟中其核醣核酸之表現量較高。綜合上述，豬之Mcl-1蛋白質結構保守性高，似與細胞凋亡之調節具有相關性。

關鍵字

豬Mcl-1 ； 互補核苷酸 ； 定量聚合酶連鎖反應 ； 組織表現

並列摘要

The cDNA sequence of the Mcl-1 protein was determined from a porcine model (Sus scrofa) and is compared to the corresponding genes of rat, human, and zebrafish. Multispecies comparison of the diversity of the Bcl-2 superfamily demonstrates Mcl-1 homology between the rat, human, and zebrafish. Porcine Mcl-1 cDNA has a size of 2487 nucleotides and encodes a polypeptide of 152 amino acids. Porcine Mcl-1 shares 83.9%, 85.3%, and 50.0% similarity to rat, human, and zebrafish Mcl-1, respectively. Real-time quantitative PCR analysis revealed that Mcl-1 transcripts are highly expressed in the uterus, lung, and liver. Despite these small molecular differences in expression, our results indicate that the structure of porcine Mcl-1 protein appears to have been remarkably conserved, and it may be involved in regulation of apoptosis.

並列關鍵字

Porcine Mcl-1 ； cDNA ； Real-time quantitative PCR ； Tissue distribution