- 期刊
Genotyping the Clonal Population Structure of a Gorgonian Coral, Junceella fragilis (Anthozoa: Octocorallia: Ellisellidae) from Lanyu, Taiwan, Using Simple Sequence Repeats in Ribosomal Intergenic Spacer
以核醣體基因區間之簡單重覆序列探討蘭嶼產白蘆莖鞭珊瑚之群體族群遺傳結構
摘要
無性生殖可使同一基因型的群體迅速占據空間，在花蟲動物的生態與進化上扮演著相當重要的角色。而欲進一步闡明群體性對區域族群結構的貢獻需要使用有效的遺傳標誌。我們設計一組增幅位於珊瑚核醣體基因區間簡單重複序列（又稱微衛星）A（C/G）（A/C）G的專一引子，並利用這組引子調查30株產自蘭嶼之白蘆莖鞭珊瑚的群體結構。放射線電泳膠片的分析結果可判斷出12條長度變異的片段，並組合出兩種不同的基因型。其中一種主要的基因型佔66.67%。數個偏低的遺傳歧異度指數（包括G0，G0:GE，和NC/N）支持蘭嶼白蘆莖鞭珊瑚的族群主要以無性生殖，而這兩種基因型可能為蘭嶼海域白蘆莖鞭珊瑚族群的奠基者。本研究證明了應用核醣體基因區間簡單重複序列解析白蘆莖鞭珊瑚在不同尺度族群解構的可行性。
並列摘要
Asexual reproduction allows clonal genotypes to dominate a space and plays an important role in the ecology and evolutionary biology of many anthozoans. To analyze the contribution of clonality to local population structure requires genetic markers that can identify individual clones (genets). We explored the possibility of developing coral-specific PCR primers to amplify a region of tetranucleotide simple sequence repeats (SSRs, also known as microsatellites) in the ribosomal intergenic spacer (IGS) region. A primer set for PCR amplification of the IGS-SSR, A(C/G)(A/C)G, was designed and applied to investigate the clonal structure of 30 colonies of the gorgonian coral, Junceella fragilis, from Lanyu (Orchid Island) off southeastern Taiwan in 1999. In total, 12 scorable bands (length variants) and 2 distinct genotypes were identified. Of the colonies sampled, 66.67% represented a single dominant genotype. Low genotypic diversity revealed by several indices (G0, G0: GE, and Nc / N) supports the scenario that the success of J. fragilis around Lanyu is likely due to vegetative propagation. The presence of 2 distinct genotypes sharing no common IGS length variants indicates that these 2 genotypes may have played the role of founders in structuring the Lanyu local population. The present study highlights the potential utility of IGS-SSR PCR techniques in delineating clonal diversity in J. fragilis populations on both local and global scales.
延伸閱讀
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