The machining accuracy plays an important role in producing a highly precise PCB router machine. However, there is little effort to study its structural behavior. To improve the machine stiffness and reduce the machining errors, a complete study of the structural analysis has been investigated. A finite element model has been generated based on the machine geometry. The FEM model was verified by conducting two experiments. One is to measure the machine mass and to compare with the calculated mass from the FEM modeling. The other is to find the dynamic behavior by conducting the modal testing experiments. In these experiments, the impulse hammer was used to measure the natural frequencies and to obtain the mode shapes. The experimental natural frequencies and mode shapes are compared with the calculated data. The results showed that they agreed with each other well. The verified FEM model was used as a tool for the structural improvement and the new machine design. This paper also illustrates the application of the structural analysis model by discussing the effect of rib thickness on the machine stiffness as an example.