應用高效液相層析法分析中藥成份層析譜峰的純度研究

The Study of Peak Purity of Chinese Medicinal Compositions by HPLC/DAD

顧巧珠(Chiao-Chu Ku) ； 鍾玉山(Yuh-Shan Chung) ； 谷皓(Hao Ku)

《藥物食品分析》 2卷4期 (1994/12) Pp. 289-296

摘要

應用光電二極體陣列檢測器一高效液相層析儀(DAD-HPLC)分析七種中藥標準品來探討分析譜峰純度，在不同濃度每一濃度打三次HPLC分析，然後兩兩相比較以求得SI值，由SI值和濃度的比較可以明顯的看出SI值會受濃度的影響。當應用參考標準品和分析譜峰的吸收光譜的比對來確認分析譜峰時，因為移動相梯度變化會影響分離管柱的平衡，造成分析譜峰滯留時間和背景(background)的變動，致使參考標準品和分析譜峰之吸收光譜比對魚法吻合，經和除基線的吸收光譜後再比較吸收光譜圖，可以得到較好的結果。本研究亦探討在HPLC分析時執行時間不足對樣品分析所造成的影響，以清氣化痰湯為例，在正常情況分析時整個執行時間是60 mim，如果將執行時間改為20 mim時連續做八次HPLC分析，可以明顯的看出hesperidin譜峰逐漸受到前次殘餘物之堆疊而影響積分面積。

並列摘要

Peak purity was studied by diode-array detector in high performance liqued chromatography (DAD-HPLC) to assay seven chinese medicinal standards which were prepared of various different concentrations. Three injections were executed for each concentration sample. then the SI values were obtained by comparing the absorption spectra of three injections of each concentration's value. The results showed that SI values increased when the concentrations decreased. The absorption spectra of peaks of reference standards and samples are usually not matched while running a gradient system. because the background of the solvent is continuously changed. However, a good match was obtained by subtracting absorption spectrum of baseline before comparing absorption spectra of reference standards and analytical peaks.
The effect of insufficient running time for HPLC analysis was also studied. For example. Ching-Shy-Huah-Tarn-Tang normally had a running time of 60 min. If the tunning time was changed from 60 min to 20 min. ther peak area was significantly interfered by the previous run during a continuous eight injections trial.

並列關鍵字

DAD-HPLC ； Peak purity