利用高效液相層析法篩檢中藥製劑中摻加之風濕鎮痛類西藥成分

Screening Chemical Drugs Used to Adulterate in Rheumatic and Analgesic Traditional Chinese Medicine by HPLC-DAD

顧祐瑞(Yoe-Ray Ku) ； 蔡明哲(Ming-Jer Tsai) ； 溫國慶(Kuo-Ching Wen)

《藥物食品分析》 3卷1期 (1995/03) Pp. 51-56

摘要

本研究利用附光二極體陣列檢出器之高效液相層析儀篩檢十七種經常於風濕鎮痛類中藥製劑中檢出之西藥成分，包括十種非類固醇類鎮痛消炎劑，acetaminophen，aminopyrine，bucetin，ethoxybenzamide，indomethacin，ketoprofen，mefenamic acid，phenylbutazone，pi-roxicam及salicylamide；二種類固醇，dexamethasone及prednisolone；二種肌肉鬆弛劑，chlormezanone及chlorzoxazone；利尿劑，hydrochlorothiazide；鎮靜安眠劑，diazepam；中樞神經興奮劑，caffeine等六類西藥成分。本法使用C18逆相層析管柱，移動相為乙腈及0.1%醋酸，利用梯度沖提，分析上述成分，效果良好，優於薄層層析法。
本研究分析82年8月至9月，由地方衛生局消費者服務中心送驗之風濕鎮痛中藥製劑三種sample A、B及C，結果sample A檢出mefenaminc acid，hydrochlorothiazide，chlorzoxazone，diazepam及caffeine西藥成分。sample B檢出mefenamic acid，indomethacin，salicylamide及hydrochlorothiazide西藥成分。sample C檢出acetaminophen，indomethacin，ethoxybenzamide，hydrochlorothiazide，chlorzoxazone，diazepam及caffeine西藥成分。

並列摘要

High-performance liquid chromatography with photodiode array detector (DAD) was developed for the identification of adulterants in rheumatic and analgesic Chinese medicine. A study was performed by using a gradient glution with acetonitrile and 0.1% acetic acid solution on a C-18 column. This method was applied to simultaneously screen 17 synthetic drugs adulterated in traditional Chinese medicine within half an hour. The drugs belonged to six pharmacological categories: antipyretic analgesics, glucocorticoids, diuretics, CNS stimulants, muscle relaxants and sedatives. They included acetaminophen, aminopyrine, bucetin, ethoxybenzamide, indomethacin, ketoprofen, mefenamic acid, phenylbutazone, piroxicam, salicylamide, dexamethasone, prednisolone, chlormezanone, chlorzoxazone, hydrochiorothiazide, diazepam and caffeine. This method can provide higher resolution and greater efficiency than thin-layer chromatography for screening adulterated synthetic drugs. Three traditional Chinese medicines sold by the dealer of Chinese natural drugs that were collected by the consumer centers of local health bureaus from August, 1993 to September, 1993 and all were examined by this method. Five synthetic drugs, mefenamic acid, hydrochlorothiazide, chlorzoxazone, diazepam and caffeine, four synthetic drugs, mefenamic acid, indomethacin, salicylamide and hydrochiorothiazide, and seven synthetic drugs, acetaminophen, indomethacin, ethoxybenzamide, hydrochlorothiazide, chlorzoxazone, diazepam and caffeine were found from Sample A, B and C, respectively.

並列關鍵字

HPLC-DAD ； Traditional Chinese medicine ； Adulterant ； Rheumatic and Analgesic