Study of PCR Detection Methods for Genetically Modified Soybeans with Reference Molecules

Hsu-Yang Lin ； Hsiu-An Wei ； Fu-Pang Lin ； Daniel Yang-Chih Shih

《藥物食品分析》 14卷2期 (2006/06) Pp. 194-202

並列摘要

The study of detection methods for genetically modified (GM) soy using certified reference material (CRM) and novel references molecules was operated on LightCycler real-time PCR machines system. The test results of this study demonstrated the methods used to be applicable to the specific quantitation of one line of GM soy. Independent repeat tests for 5, 2 and 1% CRIVI were 17, 11 and 11, and the test results mean ±SD were 4.89±0.45, 1.98±0.63 and 1.09±0.05, respectively. Series 6 repeat test by use of CRIVI and references molecules, the results of CRIVI test were 5.45±0.32, 2.42±0.13 and 1.20±0.09 (mean SD), and 4.83±0.45, 2.09±0.12 and 1.08±0.10 for references molecules testing, to 5, 2, and 1% GM content, respectively. Further, soymilk was not detected by the ELISA method at 0D450 when boiled under 70℃, 1~3 mm but high to 100℃ was detected by PCR method. Over 10 mm under 121℃, DNA highly degradation detection was more difficult. Testing results should help support the practical detection for the GM soy.

並列關鍵字

real-time PCR ； PCR ； ELISA ； reference molecules ； GMO ； soybeans ； quantification