- 期刊
An Improved Pre-culture Procedure for the Detection of Salmonella in Frozen Chicken Meat by Duplex Polymerase Chain Reaction
快速預培養流程配合雙套組聚合酶鏈反應法檢測冷凍雞肉中沙門氏桿菌
摘要
本研究結果顯示，以18小時預培養流程，並配合雙套組引子（TS5/TS11和S18/S19）聚合酶鏈反應(Polymerase chain reaction, PCR)法，可有效檢測冷凍雞肉中之沙門氏桿菌。試驗以經過或未經過冷凍處理之加菌的雞絞肉及輻射雞絞肉，採用乳糖培養液於37℃培養6 h，續以Rappaport-Vassiliadis 培養液於42℃培養12 h之預培養，再配合PCR法，均可檢測出沙門氏桿菌。應用此預培養流程檢測市售冷凍及溫體棒棒腿各50件，其結果與細菌學分析手冊(Bacteriological Analytical Manual, BAM)之檢驗結果比對，得知此2種方法無顯著差異(p≧0.05)。
並列摘要
An improved 18-hour pre-culture procedure coupling with duplex polymerase chain reaction (PCR) method capable of detecting Salmonella in frozen chicken meat is herein reported. Two pairs of primers (TS5/TS11 and S18/S19) were used for the PCR method. Positive PCR results were obtained after the 18-hour preculture (6 h in lactose broth at 37℃ followed by 12 h in Rappaport-Vassiliadis broth at 42℃) for spiked raw and irradiated meats with or without freezing treatment. The applicability of this improved procedure was tested in 50 retail frozen and 50 non-frozen chicken drumstick samples, and the results were compared with those obtained with the BAM standard culture method. No significant difference was found (p≧0.05) between these two methods.
延伸閱讀
- 陳昭蓉、劉珮如、施養志（1998）。雞肉檢體中沙門氏桿菌快速檢驗方法之探討。藥物食品檢驗局調查研究年報，()，238-249。https://doi.org/10.6946/ASRNL.199809.0238
- Chuang, S. (2020). 建構高靜水壓處理與反式肉桂醛對沙門氏菌與李斯特菌在雞絞肉中之殺菌模型 [master's thesis, National Taiwan University]. Airiti Library. https://doi.org/10.6342/NTU201904361
- Wang, S. J., Yeh, D. B., & Wei, C. I. (2009). 應用特異性聚合酶鏈反應引子快速鑑定與雞相關樣品之腸炎沙門氏菌. 藥物食品分析, 17(3), 183-189. https://www.airitilibrary.com/Article/Detail?DocID=10219498-200906-200907150041-200907150041-183-189
- 邱國皓、王金和、張聰洲、張清棟、蔡睦宗、劉振軒（2011）。以非生物素山葵過氧化酵素免疫組織化學染色法、聚合酶鏈鎖反應及原位雜合技術診斷禽類淋巴球增生性腫瘤疾病。臺灣獸醫學雜誌，37(3)，159-168。https://doi.org/10.7009/TVJ.201109.0159
- 林建谷（1998）。應用雙套式聚合酶鏈反應快速鑑定傷寒沙門桿菌。弘光學報，(32)，81-96。https://doi.org/10.6615/HAR.199810.32.06