Studies on the Analysis of Human Serum Proteins by the Gel-based Microchip and SDS-PAGE

利用微晶片電泳及聚丙烯醯胺膠體電泳分析人體血清蛋白之比較研究

謝榮峯(Jung-Feng Hsieh) ； 陳志傑(Supachok Sinchaiku) ； 歐倍鳴(Bay-Ming Ou) ； 陳水田(Shui-Tein Chen)

《藥物食品分析》 18卷6期 (2010/12) Pp. 434-439

摘要

本研究比較新型微晶片電泳與傳統聚丙烯醯胺膠體電泳分析人體血清蛋白之效能。結果顯示，血清蛋白與去醣基化血清蛋白經還原劑處理後，以微晶片生物分析儀(Bioanalyzer)搭配微晶片(Microchip)與螢光染劑進行分析，從開始到完成只需三十分鐘，而分析儀所搭配之分析軟體可自動計算樣品中蛋白質之分子量與濃度，即時呈現結果。此外，人體血清蛋白樣品也以聚丙烯醯胺膠體電泳進行分析，膠體經染色和脫色後進行影像掃描和分析，整個過程至少需要五小時。比較微晶片電泳和聚丙烯醯胺膠體電泳之分析結果，微晶片電泳即時提供精確的血清蛋白濃度，更省去了傳統電泳需染色、脫色和影像掃描等程序，有效縮短分析時間，顯示微晶片電泳可應用於臨床人體血清樣品之高通量分析。

關鍵字

微流體技術 ； 血清蛋白 ； 微晶片電泳 ； 聚丙烯醯胺膠體電泳

並列摘要

Human serum proteins can be analyzed and evaluated by using either gel-based microchip (LabChip) or traditional SDS-PAGE. However, this study concludes that a sample under reducing conditions analyzed by SDS-PAGE is more time-consuming than when using a Bioanalyzer in combination with the LabChip to perform the same task. The assay software automatically calculated the size and concentrations of serum proteins and deglycosylated serum proteins. It displayed the results in real-time, thus eliminating lengthy procedures such as imaging and analyzing. Compared to the traditional reducing SDS-PAGE, LabChip also allowed the analysis of human serum proteins with more accuracy, and a faster turnaround time. This suggested that LabChip has the potential to be used in the clinical analysis of human serum proteins.

並列關鍵字

microfluidic technology ； serum protein ； LabChip ； SDS-PAGE