Mesaconitine Plays the Major Role in the Antinociceptive and Anti-inflammatory Activities of Radix Aconiti Carmichaeli (Chuan Wu)

在川烏的鎮痛及抗發炎活性上新烏頭鹼扮演主要的作用

賴美州(Mei-Chou Lai) ； 劉怡旻(I-Min Liu) ； 劉崇喜(Shorong-Shii Liou) ； 張永勳(Yuan-Shiun Chang)

《藥物食品分析》 19卷3期 (2011/09) Pp. 362-368

摘要

本研究以川烏Radix Aconiti Carmichaeli的水萃取物，經由口服管灌方式投藥予小鼠，研究止痛反抗發炎的活性。鹿角菜膠誘導小鼠足腫脹，相當於急性發炎的模式。醋酸引起的扭體實驗和福馬林致痛反應的早期和晚期階段的小鼠皆被用来評估鎮痛活性。烏頭屬生物鹼的含量，藉由高效液相層析儀進行分析。在川烏中，烏頭鹼型生物鹼的含量順序爲新烏頭鹼＞烏頭鹼＞次鳥頭鹼。川烏水萃取物在60mg/kg，不僅有效抑制鹿角菜膠導致小鼠足腫脹，而且顯著減輕因腹腔注射醋酸所引起腹部的收縮，及足蹠下注射福馬林引起後期的疼痛反應。新烏頭鹼和烏頭鹼都能有效地減少醋酸所誘發小鼠的疼痛感覺。川烏的水萃取物由於存在高含量的新烏頭鹼，因此表現出更好的鎮痛及抗發炎作用。

關鍵字

烏頭類生物鹼 ； 鎮痛活性 ； 抗發炎活性 ； 川烏

並列摘要

This study investigated the antinociceptive and anti-inflammatory activities of the aqueous extracts of Radix Aconiti Carmichaeli (Chuan Wu) by oral gavage in mice. Carrageenan-induced paw edema served as an acute inflammation model. The acetic acid-induced writhing test and formalin-induced nociceptive responses in the early and late phases were used to assess the analgesic activity. The contents of Aconitum alkaloids were analyzed by high-performance liquid chromatography. The order of concentration of aconitine-type alkaloids in Radix Aconiti Carmichaeli was mesaconitine ＞ aconitine ＞ hypaconitine. Radix Aconiti Carmichaeli water extract at the dosage of 60 mg/kg not only effectively inhibited carrageenan-induced paw edema, hut also significantly attenuated the abdominal constriction induced by an intraperitoneal injection of acetic acid, as well as the late phase of pain response caused by a hind paw injection of formalin. Mesaconitine and aconitine are effective in decreasing the pain sensation elicited by acetic acid. The aqueous extracts of Radix Aconiti Carmichaeli exhibits antinociceptive activity and anti-inflammatory effect probably due to the presence of high content of mesaconitine.

並列關鍵字

Aconitum alkaloids ； antinociceptive activity ； anti-inflammatory activity ； Radix Aconiti Carmichaeli Chuan Wu