- 期刊
Free Radical Scavenging and Antioxidant Activities of Dorema Aitchisonii
Dorema aitchisonii之自由基清除及抗氧化活性
摘要
The aim of this study was to investigate the antioxidant properties of the aerial parts of Dorema aitchisonii by employing various in vitro assay systems including 1, 1-diphenyl-2-picryl hydrazyl radical, nitric oxide and hydrogen peroxide-scavenging activities. Fe(superscript 2+) chelating ability, reducing power and hemoglobin-induced linoleic acid peroxidation. Antihemolytic activity was investigated against H2O2 induced hemolysis in rat erythrocytes. Gallic acid, rutin, quercetin and cumarin contents of the preparation were determined by HPLC with diode array detector (HPLC/DAD). The IC50 for DPPH radical-scavenging activity was 488±18 μg/mL. The extract showed weak NO scavenging activity in the range of 0.4 and 3.2 mg/mL, as well as Fe(superscript 2+) chelating ability (IC50 = 778±22 μg/mL).The extract was effective in inhibiting hemoglobin-induced linoleic acid peroxidation, but its activity was less than Vitamin C (p ＜ 0.01). The extract was capable of scavenging H2O2 in a concentration-dependent manner with an IC50 211±11μg/mL. The extract exhibited good antihemolytic activity with an IC50 385±15μg/mL.(the corresponding value for Vitamin C being 235±9μg/mL.) The total content of phenolic compounds was 41.2±1.9 mg gallic acid equivalents/g and the total flavonoid content was 13.8±0.8 mg quercetin equivalent/g. The extract contained 7.27μg rutin and 0.011μg gallic acid/mg extract, but cumarin and quercetin were present in trace amounts. In spite of presence of phenolic and flavonoid content, especially rutin, D.aildúsonii show weak antioxidant activity, but its antihcmolytic activity was good.
並列摘要
The aim of this study was to investigate the antioxidant properties of the aerial parts of Dorema aitchisonii by employing various in vitro assay systems including 1, 1-diphenyl-2-picryl hydrazyl radical, nitric oxide and hydrogen peroxide-scavenging activities. Fe(superscript 2+) chelating ability, reducing power and hemoglobin-induced linoleic acid peroxidation. Antihemolytic activity was investigated against H2O2 induced hemolysis in rat erythrocytes. Gallic acid, rutin, quercetin and cumarin contents of the preparation were determined by HPLC with diode array detector (HPLC/DAD). The IC50 for DPPH radical-scavenging activity was 488±18 μg/mL. The extract showed weak NO scavenging activity in the range of 0.4 and 3.2 mg/mL, as well as Fe(superscript 2+) chelating ability (IC50 = 778±22 μg/mL).The extract was effective in inhibiting hemoglobin-induced linoleic acid peroxidation, but its activity was less than Vitamin C (p ＜ 0.01). The extract was capable of scavenging H2O2 in a concentration-dependent manner with an IC50 211±11μg/mL. The extract exhibited good antihemolytic activity with an IC50 385±15μg/mL.(the corresponding value for Vitamin C being 235±9μg/mL.) The total content of phenolic compounds was 41.2±1.9 mg gallic acid equivalents/g and the total flavonoid content was 13.8±0.8 mg quercetin equivalent/g. The extract contained 7.27μg rutin and 0.011μg gallic acid/mg extract, but cumarin and quercetin were present in trace amounts. In spite of presence of phenolic and flavonoid content, especially rutin, D.aildúsonii show weak antioxidant activity, but its antihcmolytic activity was good.