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Determination of Marked Components - aloin and aloe-emodin - in Aloe vera before and after hydrolysis

並列摘要


This article proposed an optimal hydrolysis condition and a chromatographic method for the analysis of polyphenol glycosides in aloe extract and its hydrolysates, The aglycones assayed were aloin and aloe-emodin, The linearity (r > 0.9997) and validation (< 10%) of the calibration curves were excellent. The amounts of aloin were 76.1 ± 5.9 and 77.7 ± 2.8 μmol/g in decoction and hydrolysate, respectively, while those of aloe-emodin were 8.3 ± 0.5 and 8.1 ± 0.3 μmol/g, The contents of aloin and aloe-emodin in aloe showed no significant difference before and after hydrolysis, This may be due to that aloe sold in Chinese medicine retailer was processed and the glycosides have already been transferred to aglycones. In addition, the components may be decomposed during processing. The method developed here can be applied to determine aglycones and their glycosides in aloe for the quality control.

並列關鍵字

aloe aloe-emodin aloin Chinese medicine hydrolysis HPLC

參考文獻


Chiang, H. M., Yeh, Y. R., Chao, P. D. L., Hsiu, S. L., Hou, Y. C., Chi, Y. C. and Wen, K. C. 2005. Metabolic pharmacokinetics of isoflavones in the roots of Pueraria lobata in rats. Mid. Taiwan J. Med. 10: 57-64.
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Wintola, O. A., Sunmonu, T. O. and Afolayan, A. J. 2010. The effect of Aloe ferox Mill. in the treatment of loperamide-induced constipation in Wistar rats. BMC Gastroenterol. 10: 95-100.
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Grindlay, D. and Reynolds, T. 1986. The Aloe vera phenomenon: a review of the properties and modern uses of the leaf parenchyma gel. J. Ethnopharmacol. 16: 117-151.

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