Colonization Capability of Fluorescent Pseudomonads in the Rhizosphere of Tomato

螢光假單胞菌在番茄根圈之群集能力

陳金枝(Chin-Chih Chen) ； 徐世典(Shih-Tien Hsu) ； 曾國欽(Kuo-Ching Tzeng)

《植物病理學會刊》 1卷3期 (1992/09) Pp. 130-139

https://doi.org/10.6649/PPB.199209_1(3).0004

摘要

由番茄根部分離的螢光假單胞菌菌株在番茄根圈之群集能力差異甚大，供試64個菌株被覆於番茄種子後，其在播種21天後的幼苗根圈之群集量從0(偵測不到)至5.52 log cfu/g wet root，而大多數菌株約在3-4 log cfu/g wet root左右。一個群集能力較佳的菌株FP10被覆於番茄種子後，於溫室及田間情況下其在根圈之族群密度均隨番茄生長期的增長而遞減，且在根部之分布大多集中於連接莖處附近；種子被覆再加上幼苗移植前根部浸漬FP10菌株可提高該菌在根圈之群集量及延使其在根部的分布。以種子被覆細菌法處理，FP10菌株在番茄根圈之菌量均隨著處理濃度的增加而提高。於低溫(20 C)或經高溫高壓滅菌之土壤環境中，FP10菌株在根圈之族群密度及在根上之分布較高溫(28 C)或未經滅菌之自然土中爲高及廣。FP10菌株在種植於台東坋質壤土、大南壤土、大里壤砂土及大里砂質壤土中的番茄根圈之群集能力差異不大，惟在較酸性(pH 4.1)的大溪壤土中，其根圈之族群密度較低且降低亦較快。大溪壤土的pH値由4.1提高至5.3-7.1可顯著增加菌株在根圈之族群密度。

關鍵字

番茄 ； 螢光假單胞菌 ； 根圈群集

並列摘要

Strains of fluorescent pseudomonads isolated from tomato roots differed markedly in their rhizosphere colonization ability. The rhizosphere population of 64 strains tested on 21-day-old tomato plants following seed bacterization ranged from 0 (nondetectable) to 5.52 log cfu/g wet root, with most strains in the range of 3-4 log cfu/g wet root. The rhizosphere population of strain FP10 (a better rhizosphere colonizer) declined with plant growth and colonized mostly sections of roots near the point of attachment with the stem under greenhouse and field conditions. Seed bacterization followed by root treatment before transplanting increased rhizosphere populations of strain FP10 and enhanced also the distribution of the strain along the root. With seed bacterization, an increase in inoculum levels resulted in a proportionatc increase of strain FP10 colonizing the rhizosphere. Surain FP10 had a higher density of rhizosphere population and colonized more root segments in the autoclaved soil than in the nonautoclaved soil. The rhizosphere colonization of strain FP10 was greater at 20 C than at 28 C Colonization of strain FP10 in the rhizosphere of tomato growing in four of five soils tested was similar but in an acidic Tachi loam soil, the rhizosphere population decreased more rapidly than in the other four soils. Adjustment of pH in Tachi soil from the original 4.1 to a range of 5.3-7.1 increased significantly the rhizosphere population of strain FP10.

並列關鍵字

tomato ； fluorescent pseudomonads ； rhizosphere colonization