- 期刊
絲核菌之細胞核螢光染色
Nuclear Staining of Rhizoctonia spp. with Fluorescence dyes
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並列摘要
Young hyphae of Rhizoctonia spp. grown from potato dextrose agar plug on glass slides were used to stain with fluorescent dyes for nuclei and septa. Nuclei and septa were stained very clearly and easily obserable with 4,6'-diamidino 2-phenyl-indole (DAPI) in pH 7 buffer and Acridine orange in pH 10 buffer. When stained with DAPI, Fluorescent brightener 28 could be used for double staining, which increased the efficiency of cell wall and septa staining. This technique was very simple, and could finish staining within a short period of time.
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