Comparison of Diagnostic Hosts and Serological Tests for Four Cucurbit Potyviruses

四種屬於PVY群之瓜類病毒在診斷寄主與血清反應特性之比較

黃秋雄(Chiou-Hsiung Huang) ； 梁素琴(Suh-Chyn Liang) ； 鄧汀欽(Ting-Ching Deng) ； 許秀惠(Shiow-Huey Hseu)

《植物病理學會刊》 2卷3期 (1993/09) Pp. 169-176

https://doi.org/10.6649/PPB.199309_2(3).0008

摘要

四種病毒包括矮南瓜黃化嵌紋病毒(zucchini yellow mosaic virus，ZYMV)，木瓜輪點病毒-西瓜系統(type W isolate of papaya ringspot virus﹐PRV-w)，西瓜嵌紋病毒-2(watermeIon mosaic virus-2，WMV-Z)及甜瓜脈綠嵌紋病毒(melon vein-banding mosaic virus，MVbMV)。從供試的六種診斷寄主反應結果獲知，PRV-W不感染Cucumis metuliferus＂PI 292190＂，但其他三種病毒可。Phaseolus vulgaris ＂Black Turtle 2＂和Nicotiana benthamiana僅被WMV-2感染。利用Luffa cylindrica為診斷寄主，ZYMV可引起嚴重嵌紋與葉片皺縮之病徵，MVbMV引起輕微嵌紋，PRV-W引起輕微嵌紋或潛伏，但WMV-2不會感染該植物。另外一種寄主，Lagenaria siceraria﹐如果感染MVbMV則產生明顯嵌紋病徵，而PRV-W僅引起輕微嵌紋或潛伏感染，該植物不被WMV-2或ZYMV感染。從目前所得結果，MVbMV可利用Chenopodium quinoa分開，因為MVbMV在該植物上產生系統性單斑，而其他三種病毒僅在接種葉形成單斑。在血清反應特性上，四種病毒之抗血清(antisera to virus particles)在SDS-immunodiffusion tests上均可區別之。若以內含體抗血清(antisera to cylindrical inclusion (CI))，同樣可以分別出。然MVbMV CI抗血清會與WMC-2反應，但有交叉現象(spur precipitin lines)。利用ELISA tests，抗體與同源抗原(homologous antigen)反應之ELISA吸收值相當高，對不同毒抗原均不反應。因此同樣可以利用ELISA將四種不同病毒區別之。然而相同病毒之不同分離株或系統(virus isolate/strain)，不論利用SDS-immunodiffusion test或ELISSA，反應結果可能略有差異，必須視isolate或strain作進一步之研究。

關鍵字

瓜類PVY病毒

並列摘要

Four cucurbit potyviruses, i.e. zucchini yellow mosaic virus (ZYMV), type W isolate of papaya ringspot virus (PRV-W), watermelon mosaic virus-2 (WMV-2) and melon vein-banding mosaic virus (MVbMV), were compared by their reactions on six diagnostic hosts, SDS-immunodiffusion and ELISA tests. Results of host reactions indicated that isolates of PRV-W group (PRV-W FL, PRV-W T and PRV-Wv) did not infect Cucumis metuliferus PI ＂292190＂, but other three viruses did. Both Phaseolus vulgaris ＂Black Turtle 2＂ and Nicotiana benthamiana were only infected by WMV-2. In Luffa cylindrica, ZYMV induced severe mosaic and rugose symptoms, MVbMV mild mosaic, PRV-W latent infection or mild mosaic, while WMV-2 did not infect this plant. Another diagnostic host, Lagenaria siceraria, generally produced conspicuous mosaic symptoms when infected with MVbMV but latent or mild mosaic when infected with isolates of PRV-W group. This plant did not react to WMV-2 and ZYMV. Chenopodium quinoa separated MVbMV from other three viruses by producing systemic local lesions in contrast to localized lesions of other viruses. In SDS-immunodiffusion tests, antisera to virus particles (VP) of each virus were specifically reactive to their homologous antigens. However, antiserum to PRV-W FL VP did not react with PRV-Wv. Conversely, antiserum to PRV-Wv VP produced spur precipitin bands with PRV-W FL. Antisera to cylindrical inclusion (CI) proteins were specific for the homologous viruses except that antiserum to MVbMV CI which produced spur precipitin lines with WMV-2 antigen. In ELISA tests, antiserum to PRV-W FL VP did not detect PRV-Wv, and antiserum to PRV-Wv VP reacted only weakly with PRV-W FL antigen when compared with the homologous antigen/antibody reactions.

並列關鍵字

Cucurbit potyvirus ； ZYMV ； PRV-W ； WMV-2 ； MVbMV.