Application of immunofluorescent Staining and Tissue-blotting Techniques for the Detection of a Mycoplasmalike organism Associated with Sweetpotato Witches' Broom

利用免疫螢光染色及組織轉印技術偵測甘藷簇葉病病原似菌質體

沈偉強(Wei-Chang Shen) ； 林長平(Chan-Pin Lin)

《植物病理學會刊》 3卷2期 (1994/06) Pp. 79-83

https://doi.org/10.6649/PPB.199406_3(2).0001

摘要

利用單元抗體將免疫螢光染色及組織轉印技術應用於甘藷簇葉病(SPWB)病原似菌質體(MLO)之偵測及鑑別上。利用此二種血清技術可將SPWB-MLO與引起泡桐、絲瓜、Ipomoea obscura等簇葉病，翠菊、白楊黃化病及水稻黃萎病等病原似菌質體予以鑑別區分。SPWB-MLO亦被證實與花生、菜豆、小葉括根及山地豆等簇葉病之病原似菌質體有血清相關性。利用此二種血清技術可精確的偵測出在SPWB罹病甘藷及日日春之根、莖、及葉部中肋等組織切片中之SPWB-MLC，此外在罹病日日春之花托及子房基部亦可偵測到SPWB-MLO。

關鍵字

單元抗體 ； 血清偵測 ； 組織轉印技術

並列摘要

immunnofluorescent staining and tissue blotting techniques were applied in the detection and differentiation of a mycoplasmalike organism (MLO) associated with sweetpotato witches' broom (SPWB) with monoclonal antibodies. SPWB-MLO was differentiated from MLOs associated with paulownia, loofah, and Ipomoea obscura witches' brooms, aster and ash yellows, and rice yellow dwarf in both serological tests. SPWB-MLO was also proved to be serologically related to peanut and asparagus bean witches' broom MLOs, and MLOs associated with witches' broomed weeds, Rhynchosia minima and Alysicarpus vaginalis. Using both techniques, SPWB-MLO was readily detected in tissue sections of roots, stems, and leaf midribs prepared from diseased sweetpotato and periwinkle, and also in sections of the fleshy receptacle and the basal part of the ovary prepared from diseased periwinkle.

並列關鍵字

monoclonal antibody ； immunodiagnostics ； tissue-blotting techniques