並列摘要
Monosporascus cannonballus produces a single ascospore per ascus, but no conidial stage has been observed. Thick and short hyphal cells were found in vegetative mycelia grown on PDA for one month. It was rare to see ascospore germination of M. cannonballus. The percentage of ascospore germination of the isolate MC-E3 was lower than 0.59%. The pathogen was grown on a sand-rice chaff medium for 30 days which was used as an inoculim (160 ascospores/g medium) in the following experiments. Inoculum mixed with soil was put on the outdoors. The survival ability off the pathogen was evaluated bimonthly in the greenhouse by direct seeding the muskmelon seeds into the artificial infested soil. M. cannonballus c o u l d cause root rot on muskmelon seedlings within 10 months after the infested soil incubated at outdoor. Reisolation of the pathogen from diseased roots also showed that the inoculum could survive in the infested soil for 10 months. Diseased roots of dry and undecomposed muskmelon caused by the fungus was collected from the field. It was found that M. cannonballus could be isolated from diseased tissues after 1.5 months incubation. The ability of vegetative mycelium on diseased roots to act as inoculum was lower than ascospores.
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