Amplification of virD1 and opine synthase genes from tumorigenic rose and aster strains of Agrobacterium in Taiwan by polymerase chain reaction

陳昭瑩(C. Y. Chen) ； 廖惠玲(H. L. Liao) ； 鍾瑞洲(J. C. Chung)

《植物病理學會刊》 8卷4期 (1999/12) Pp. 143-148

https://doi.org/10.6649/PPB.199912_8(4).0003

摘要

本研究測試以聚合酵素連鎖反應(polymerase chain reaction, PCR)擴增玫瑰及紫花宿苑癌腫病菌vir D1及opine合成酵素基因的效果。在供試臺灣菌株中，僅分離自玫瑰病株的癌腫病菌可以獲得vir D1基因的PCR產物，而分離自紫花宿苑的癌腫病菌則無法擴增出預期的PCR產物。在opine合成酵素基因的擴增試驗，分離自玫瑰病株的癌腫病菌可以獲得agrocinopine合成酵素基因片段的PCR產物；分離自紫花宿苑的癌腫病菌則可擴增得到octopine合成酵素基因的PCR產物。根據本研究結果推測癌腫病菌vir D1基因的核酸序列可能在不同群的菌系呈現某種程度的差異性。此外，癌腫病菌opine合成酵素基因的類型可能藉由聚合酵素連鎖反應即可以快速地鑑定。

關鍵字

聚合酵素連鎖反應 ； agrocinopine合成酵素基因 ； nopaline合成酵素基因 ； octopine合成酵素基因

並列摘要

We tried to amplify the vir D1 gene and opine synthase genes from tumorigenic strains of Agrobacterium in Taiwan by polymerase chain reaction (PCR). The vir D1 gene was amplified from tumorigenic strains of Agrobacterium isolated from rose gall tissues; but could not be amplified from tumorigenic strains of Agrobacterium isolated from aster gall tissues. In the PCR analysis of three opine synthase genes, the segment of agrocinopine synthase gene was amplified from the tumorigenic rose strains whereas the octopine synthase gene was amplified from tumorigenic aster strains. None of these tumorigenic strains could generate PCR product with oligonucleotide primers for nopaline synthase gene. These results implicate that the sequence of vir D1 virulence gene may be distinct among different groups of phytopathogenic Agrobacterium. In addition, the type of tumor-inducing plasmid may be quickly determined by PCR amplification of opine synthase gene(s) from the agrobacterial cell.

並列關鍵字

polymerase chain reaction (PCR) ； vir D1 ； agrocinopine synthase gene ； nopaline synthase gene ； octopine synthase gene