線蟲蟲齡對Meloidogyne javanica及Pratylenchus coffeae侵入紅豆與蘿蔔苗根部速率之影響

Effect of Nematode Age and Plant Species on the Penetration Speed of Meloidogyne Javanica and Pratylenchus coffeae

蔡碧雲( B. Y Tsai)

《植物病理學會刊》 10卷2期 (2001/06) Pp. 65-70

https://doi.org/10.6649/PPB.200106_10(2).0002

摘要

蘿蔔汁(稀釋比例1:2)可100%抑制Meloidogyne javanica及Pratylenchus coffeae對紅豆根部之侵入，以之作為侵入抑制劑，有助於對線蟲侵入速度之研究。結果發現線蟲的培養與保存時間及植物種類皆對於M. javanica及P. coffeae之侵入速度有影響。M. javanica保存於15℃下平均為26天之二齡幼蟲侵入速度較平均保存僅3.5天者慢，且最終之侵入率僅約為後者之四分之一。保存時間相同之二齡幼蟲侵入紅豆根部之速度比侵入蘿蔔苗者快，但最終之侵入率沒有差異。由於實驗用的P. coffeae線蟲並非由卵塊孵化，其蟲齡難以單獨計算，故以整個Gamborg's B5平板上之線蟲的培養天數為代表，稱為培養齡。P. coffeae之培養齡30天者侵入紅豆苗根部之速度比培養齡63天及96天者快，且最終侵入率也較高。P. coffeae對紅豆苗根部之侵入速度比對蘿蔔苗者快。兩種線蟲皆可在接種後立即侵入紅豆苗根部。接種後60分鐘，M. javanica已有9.4%之線蟲侵入，而P. coffeae有 7.0%侵入。

關鍵字

侵入速度 ； 侵入率 ； 染色

並列摘要

Radish juice (Dilution factor l: 2 (w/v) was found to be 100 % effective on inhibiting the penetration of Meloidogyne. Javanica and Pratylenchus coffeae on the roots of Adzuki bean seedlings. Using it as a Penetration-inhibiting agent greatly facilitated the research on the penetration speed of nematodes. Both storage and cultivation times of nematodes and plant species were found to affect the penetration speed of M. javanica and P. coffeae. The penetration speed of the second-stage juveniles of M. javanica, after stored at 15℃ for 26-days, was much slower than those of juveniles stored for 3.5 days, and the final penetration rates (%) of the former were only approximately one fourth of the latter. The juveniles stored for the same periods of times penetrated into the roots of Adzuki bean much faster than into the roots of radish, but the final penetration rates were not significantly different. Due to the difficulty in recording the age of individual P. coffeae, the nematode age was represented by the number of days of the whole population cultured on excised roots in Gamborg’s B5 medium and was termed culture-age. The penetration speed and the final penetration rate of P. coffeae nematodes at the culture-age of 30-days were faster and higher than those of nematodes cultivated for 63-days and 96-days on the roots of Adzuki bean. P. coffeae penetrated into the roots of Adzuki bean faster than into the roots of radish. Both M. javanica and P. coffeae were capable of penetrating into the roots of Adzuki bean immediately after inoculation. There were 9.4% of M. javanica and 7.0% of P. coffeae penetrated into the roots 60 minutes after inoculation.

並列關鍵字

nematode age ； Meloidogyne javanica ； Pratylenchus coffeae ； penetration speed ； penetration rate ； staining