黑眼豇豆嵌紋病毒台灣分離株鞘蛋白基因與3' 端非轉譯區序列之解讀與分析

Analysis of the Sequence of Coat Protein Gene of Blackeye cowpea mosaic virus in Taiwan

陳許玉鈴( Y. L Chen-Hsu) ； 王惠亮( H. L Wang)

《植物病理學會刊》 10卷4期 (2001/12) Pp. 165-172

https://doi.org/10.6649/PPB.200112_10(4).0003

摘要

黑眼豇豆嵌紋病毒(Blackeye cowpea mosaic virus，BlCMV)屬於長絲狀的馬鈴薯Y病毒屬(Potyvirus)病毒，為豆科作物最重要病毒之一。利用反轉錄聚合?連鎖反應選殖黑眼豇豆嵌紋病毒之3'端區域，經譯讀後顯示其鞘蛋白基因共有861個核?酸，可轉譯成287個胺基酸，3'端非轉疫區部分則具有251個核?酸。由於鞘蛋白基因及3'端非轉譯區序列可作為Potyvirus屬鑑別與分類之標準，故本研究之主要目的為，藉分子層次上之比對，以明瞭BlCMV與幾種已發表的bean common mosaic subgroup病毒間之親緣性。比對結果顯示，台灣本地之BlCMV-TW分離株與國外報告之BlCMV-F(佛羅里達)、B1CMV-W、CAbMV-TH(泰國)、AzMV-H(日本)、PStV-ChinaG(中國)、PStV-B(美國)、BCMV-NY15(美國)、BCMV-NL1及BCMV-NL4彼此親緣關係極為接近，彼此之間相同度皆大於90%。因此這些不同地區報告之病毒應屬於同一病毒(B1CMV)的不同品系。其中台灣本地之B1CMV-TW鞘蛋白基因序列與B1CMV-F及CAbMV-TH具較高之相同度，與PStV-B及PStV-ChinaG具較低之相同度。而B1CMV之3’端非轉譯區則與B1CMV-W具有較高之相同度，與PStV-B具較低之相同度。

關鍵字

黑眼豇豆嵌紋病毒 ； 鞘蛋白基因 ； 核?酸序列

並列摘要

Blackeye cowpea mosaic virus (B1CMV) is a member of the genus Potyvirus and is one of the most important virus infecting leguminaceous crops. In this study, the cDNAs of the 3'-terminal regions of B1CMV-TW was amplified by RT-PCR and cloned. The nucleotide and amino acid sequences of coat protein (CP) gene and 3' non-translated region (NTR) of B1CMV-TW was analyzed and compared with other reported members of bean common mosaic virus subgroup (B1CMV-F、B1CMV-W、CabMV-TH、AzMV-H、PStV-B、BCMV-NY15、BCMV-NL1 and BCMV-NL4)from different geographic areas. The results showed that the sequence of 1112 bp located in the 3’terminal regions of BICMV-TW including 861 nucleotides of CP gene encoding a 287 amino acid which was followed by a non-translated region of 251 nuc1eotides and a 3' poly(A) tail. Comparison of BlCMV-TW with other reported members of bean common mosaic virus subgroup indicated that they are all the same virus but different strains. The results also showed that CP gene of BlCMV-TW had a higher identity with BICMV-F and CAbMV- TH, and a lower identity with PStV-B and PStV-China G. The 3' non-translated region of BICMV-TW has a higher identity with BlCMV-W, and a lower identity with PStV-B.

並列關鍵字

Blackeye cowpea mosaic virus ； coat protein gene ； nucleotides sequence