齒舌蘭輪斑病毒台灣系統基因序列譯讀與分析

Molecular Sequencing and Analysis of the Viral Genome of Odontoglossum Ringspot Virus Taiwan Strain

王惠亮(H. L. Wang) ； 王志農(J. N. Wang) ； 張清安(C. A. Chang)

《植物病理學會刊》 13卷2期 (2004/06) Pp. 97-106

https://doi.org/10.6649/PPB.200406_13(2).0002

摘要

齒舌蘭輪斑病毒(Odontoglossum ringspot virus, ORSV)，是感染蘭科植物最重要的病毒之一，在分類上屬於菸草嵌紋病毒屬(genus Tobamovirus)，是一種長桿狀顆粒病毒，其顆粒大小約為長度300nm，寬度18nm，顆粒中軸可清楚分辨有溝狀構造。在地理的分佈上遍佈溫帶及熱帶地區的非洲、亞洲洲、歐洲、北美及南美洲。本研究完成選與譯齒舌蘭輪斑病毒台灣系統(ORSV-TW)的全長度核苷酸和胺基酸序列，並與已報告之ORSV-S1(新加坡)、ORSV-Cy1(日本)、ORSV-(韓國)及ORSV-(美國)等四個系統作全長度核苷酸和胺基酸序列進行比對與分析。ORSV-TW 全長度核苷酸序列為6612個核苷酸(accession number: AY571290), 其中126/183 kDa RNA-dependent RNA polymerase (RdRp) 基因共3336/4839 個核苷酸，可分別轉譯成1112個胺基酸，分子量為126kDa之 replicase protein 和1612個胺基酸，分子量為183 kDa之readthrough protein、movement protein(MP)基因共912個核苷酸，可轉譯成303個胺基酸，分子量為33 kDa、基因(CP)共477個核苷酸，可轉譯成158個胺基酸，分子量為18 kDa、5’端非轉譯區63個核苷和3’端非轉譯區414個核苷酸。經與國外報告ORSV核苷酸序列相同度及胺基酸序列相似度比對結果顯示，五個系統間各基因的比較結果都達到96%以上，顯示五者具高同源性，種內的遺傳歧異度相當低，為同種病毒之不同系統。

關鍵字

齒舌蘭輪斑病毒 ； 開放轉譯架構 ； 核苷酸序列

並列摘要

Odontoglossum ringspot virus (ORSV) is a member of Tobamovirus genus in the family of Tobamoviridae. It is an important virus infecting orchid plants and occurring worldwide. The purpose of this study is to sequence the genomic regions of ORSV Taiwan strain (ORSV-TW), and to compare its nucleotide and amino acid sequences with that of four reported ORSV strains of various origins, i.e. ORSV-S1 (Singapore), ORSV-Cy1 (Japan), ORSV-Cy (Korea) and ORSV-U (America). The full length genomic RNA of ORSV-TW were 6,612bp (accession number: AY571290). The length of nucleotide sequences for 126/183 kDa RNA-dependent RNA polymerase (RdRp) genes were 3,336 and 4,839 bp and encoded RdRp replicase and readthrough protein, respectively. Genes of movement protein (MP) and coat protein (CP) consisted of 909 and 474 nucleotides and encoded proteins of MW of 33 and 18 kDa, respectively. The nucleotide sequences of 5’ and 3’ non-translated regions (NTR) were 63 and 414 bp, respectively. The results of comparison between nucleotide and amino acid sequences of ORSV-TW with that of those reported ORSV strains showed more than 96% identity and similarity indicating that they are different strains of the same virus.

並列關鍵字

Odontoglossum ringspot virus ； open reading frame ； nucleotide sequence