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台灣地區葡萄園鞘線蟲Hemicriconemoides parasinensis (Nematoda: Criconematoidea, Criconematidae)之鑑定

摘要


自2007年6月起,從台灣葡萄專業區的根圈土壤中分離鑑定4個種群Hemicriconemoides parasinensis Chen & Liu 2003,此一鞘線蟲爲台灣地區的新記錄種,且爲葡萄作物上的首次發現。各鞘線蟲種群之鑑定依據包括光學和電子顯微鏡(SEM)的形態特徵觀察和形態測量值(morphometrics)比對,並輔以包括完整5.8S基因、內轉錄區間ITS-1和ITS-2及分18S和28S基因的核糖體DNA(ribosomal DNA, rDNA)片段序列相同度比對分析。本研究爲首次在GenBank中登錄H. parasinensis的rDNA片段代表性序列,序列大小爲1052bp,其中ITS-1、ITS-2及5.8S的序例依序分別爲378bp、218bp及156dp。

並列摘要


Four populations of Hemicriconemoides spp. have been isolated by modified Baermann funnel method from the rhizosphere soil of grape plants from 4 plantations in Taiwan since June, 2007. Comparisons of the morphology, morphometrics and ribosomal DNA fragment sequences (including full 5.8S gene, ITS-l, ITS-2 and partial 18S and 28S gene) have identified the 4 populations as H. parasinensis Chen & Liu 2003. H. parasinensis was the first report in Taiwan. The representative sequences of rDNA fragment of H. parasinensis was 1052 bp, and were submitted firstly to GenBank.

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