The purpose of this article is to study the phenomenon of the rear-body separation based on results from numerical simulation. At first, the equation of separation is derived to relate separation velocity to the drag forces acting on the front and rear bodies. Secondly, the drag forces are evaluated by solving the turbulent flow fields, enclosing the separation gap, based on the quasi-steady assumption. These drag forces are applied to analyzing separation distance through the derived separation equation. Present study reveals that the motion characteristics of the front and rear bodies, including drag forces, flight Mach number, and initial separation velocity, all have great influence on separation performance. Although quasi-steady assumption is used in present computations, the transient effect on separation distance is worth further studying.