銦-111-DTPA-Octreofide 之放射化學純度分析方法比較與金屬離子含量對於銦-111-DTPA -Octreofide 之標幟效率影響研究

Comparison of Methods to Determine Radiochemical Purity and the Effects of Metallic Ions on the Labeling efficiency of (superscript 111)In-DTPA-Octreotide

夏建忠(Chien-Chung Hsia) ； 林武智(Wuu-Jyh Lin) ； 黃芙蕾(Fu-Lei Hwang) ； 沈立漢(Lie-Hang Shen) ； 蘇瑀(Yeu Su) ； 吳榮燦(Rong-Tsun Wu) ； 傳應凱(Ying-Kai Fu)

《核子醫學雜誌》 17卷4期 (2004/12) Pp. 191-196

https://doi.org/10.6332/ANMS.1704.002

摘要

Background: Somatostatin, a naturally occurring 14-amino acid peptide, can be thought of as an anti-growth hormone and functional down-regulator of sensitive tissue. Somatostatin receptor scintigraphy (SRS) with the diethylenetriamino-pentaacetic acid conjugated somatostatin analogue [(superscript 111)In-DTPA-D-Phe1] Octreotide, also known as (superscript 111)Inpentetreotide (OctreoScan), approved for clinical use by the Food and Drug Administration in June1994, have been of considerable value in scintigraphically identifying various neuroendocrine tumors. It is a new non-invasive modality for the evaluation of tumors expressing receptors for somatostatin. These receptors are presented on neuroendocrine. In oncology SRS is a promising diagnostic tool for localizing primary tumors, staging, control and follow-up after therapy. The purposes of this study were to evaluate a new ITLC-SG approach for determination of radiochemical purity, and to evaluate the effects of metallic ions on the labeling efficiency of (superscript 111)In-DTPA-Octreotide.
Methods: To evaluate the ITLC-SG method by comparing the results radiochemical purity analysis with the methods of cartridge and HPLC. Adding Cu(superscript 2+), Fe(superscript 3+)and In(superscript 3+) ions in (superscript 111)In-indium chloride solution to evaluate the effects of these cations on the labeling efficient of (superscript 111)In-DTPA-Octreotide.
Results: The relation coefficient among the three methods was ＞0.99. Significant reductions in the labeling efficient of (superscript 111)In-DTPA-Octreotide were observed with Cu(superscript 2+) Fe(superscript 3+) and In (superscript 3+) ions of 10, 20 and 5 ppm, respectively, in (superscript 111)In-indium chloride solution.
Conclusion: The ITLC-SG method could be used as an alternative to determine the radiochemical purity of (superscript 111)In-DTPA-Octreotide routinely. The metallic impurities in (superscript 111)In-indium chloride may reduce the labeling efficiency of (superscript 111)In-DTPA-Qctreotide.

關鍵字

銦-111-DTPA-Octreotide ； 放射化學純度分析 ； 金屬離子

並列摘要

Background: Somatostatin, a naturally occurring 14-amino acid peptide, can be thought of as an anti-growth hormone and functional down-regulator of sensitive tissue. Somatostatin receptor scintigraphy (SRS) with the diethylenetriamino-pentaacetic acid conjugated somatostatin analogue [(superscript 111)In-DTPA-D-Phe1] Octreotide, also known as (superscript 111)Inpentetreotide (OctreoScan), approved for clinical use by the Food and Drug Administration in June1994, have been of considerable value in scintigraphically identifying various neuroendocrine tumors. It is a new non-invasive modality for the evaluation of tumors expressing receptors for somatostatin. These receptors are presented on neuroendocrine. In oncology SRS is a promising diagnostic tool for localizing primary tumors, staging, control and follow-up after therapy. The purposes of this study were to evaluate a new ITLC-SG approach for determination of radiochemical purity, and to evaluate the effects of metallic ions on the labeling efficiency of (superscript 111)In-DTPA-Octreotide.
Methods: To evaluate the ITLC-SG method by comparing the results radiochemical purity analysis with the methods of cartridge and HPLC. Adding Cu(superscript 2+), Fe(superscript 3+)and In(superscript 3+) ions in (superscript 111)In-indium chloride solution to evaluate the effects of these cations on the labeling efficient of (superscript 111)In-DTPA-Octreotide.
Results: The relation coefficient among the three methods was ＞0.99. Significant reductions in the labeling efficient of (superscript 111)In-DTPA-Octreotide were observed with Cu(superscript 2+) Fe(superscript 3+) and In (superscript 3+) ions of 10, 20 and 5 ppm, respectively, in (superscript 111)In-indium chloride solution.
Conclusion: The ITLC-SG method could be used as an alternative to determine the radiochemical purity of (superscript 111)In-DTPA-Octreotide routinely. The metallic impurities in (superscript 111)In-indium chloride may reduce the labeling efficiency of (superscript 111)In-DTPA-Qctreotide.

並列關鍵字

(superscript 111)In-DTPA-Octreotide ； radiochemical purity analysis ； metallic ions