- 期刊
Validation of an Animal FDG PET Imaging System for Study of Glioblastoma Xenografted Mouse and Rat Models
氟化去氧葡萄糖正子電腦斷層掃描評估神經膠質瘤小鼠及大鼠模式之研究
摘要
背景：由於神經膠質瘤的高發生率與預後不易，若能發展出有效的早期診斷工具將具有重要的臨床意義。本研究目的爲建立動物用正子電腦斷層掃描(PET)配合氣－18-氟化去氧葡萄糖(FDG)作爲監測動物腦部神經膠質瘤生長的工具，研究對象分爲小鼠與大鼠兩種品系。 方法：使用DBTRG-05MG與RG2兩種神經膠質瘤細胞，分別植入小鼠與大鼠顱內，品系各爲SCID與Wistar。植入腫瘤的動物靜脈注射FDG後待1-2小時，進行動態(dynamic)FDG-PET掃描以求得的腦部最佳造影時間點；其後分別於第7、l0、14與17天進行腫瘤影像追蹤。 結果：腫瘤最小可偵測的限值爲2.5 mm。最佳造影時間即腫瘤攝取FDG與背景組織差異最大的時間點，小鼠爲藥物注射後40分鐘，大鼠爲90分鐘。腫瘤攝取FDG的程度也隨著腫瘤成長而明顯增加。 結論：本研究已成功探討FDG-PET用於監測大、小鼠腦部神經膠質瘤生長的最適條件，此結果將有助於未來更多相關動物研究的進行。
並列摘要
Introduction: Because of the high incidence and poor prognosis of gliomas, the development of pre-clinically effective diagnostic tools is of great importance. The objective of this study is to validate the use of FDG PET imaging system for monitoring glioma proliferation in two rodent models. Methods: Two kinds of glioblastoma cells (human DBTRG-O5MG and rat RG2 tumor cells) were implanted intracerebrally to SCID mice and Wistar rats, respectively. To characterize the optimal scanning time required for effective detection of brain tumors, dynamic animal PET were acquired for 1 and 2 h immediately after intravenous injection of the FDG radiotracer to mice and rats, respectively. Test animals were then subjected to serial animal PET scans at day 7, 10, 14, and 17 after tumor cell implantation. Results: Mouse and rat brain tumors were first detected by FDG micro PET imaging at day 7 and 10 after tumor implantation, respectively. The smallest tumor size detectable was 2.5 mm in diameter. The peak tumor-to-background ratio was observed at 40 mm post-injection in the mouse model and at 90 mm post- injection in the rat study. Both the peak standard uptake value of FDG and the tumor-to-background ratios were found to increase as the tumors grew over time. Conclusion: A FDG PET scan protocol was validated for detecting and monitoring glioma tumor growth in both mouse and rat models. Optimal FDG uptake period required and optimal scanning times for experimental tests were hence established for future systematic studies in relevant animal models.
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