利用Clostridium acetobutylicum生產生質丁醇之醱酵效能研究

A Study on Fermentative Production of Biobutanol by Clostridium acetobutylicum

林忠亮(Chung-Liang Lin) ； 車佩怡(Pei-Yi Che) ； 羅泳中(Yung-Chung Lo) ； 張嘉修(Jo-Shu Chang)

《石油季刊》 46卷4期 (2010/12) Pp. 75-84

https://doi.org/10.30128/JP.201012.0006

摘要

本研究針對兩株篩選自土樣的丁醇生產菌進行生質丁醇醱酵效能研究。首先以16S rDNA基因序列比對的方式，鑑定此兩菌株屬於Clostridium acetobutylicum菌株，分別命名為Cl. acetobutylicum S1與Cl. acetobutylicum S2。接著，測試此兩菌株之丁醇醱酵效能。搖瓶測試結果顯示，兩株菌均能利用葡萄糖為碳源，進行丁醇之生產；其中，以Cl. acetobutylicum S1有較佳之丁醇生產表現，其丁醇產量濃度達7.5g/L、丁醇生產速率為0.25g/L-h、而丁醇產率則為0.41mol butanol/mol glucose。伴隨丁醇之生成，其他代謝產物如乙醇、丙酮、丁酸與乳酸亦隨之產生，然以丁醇生產為主。至於S2菌株，其葡萄糖之轉化率與丁醇產量皆略低於Cl. acetobutylicum S1。此兩株菌之丙酮：丁醇：乙醇產量比相當接近，分別為2.76:22.7:1(Cl. acetobutylicum S1)與2.77:22.9:1(Cl. acetobutylicum S2)。在醱酵槽規模放大培養時，Cl. acetobutylicum S1之最大丁醇產量達14.2g/L且丁醇產率亦可提升至0.60mol butanol/mol glucose。此結果顯示，在條件容易控制的醱酵槽中，Cl. acetobutylicum S1有較佳的丁醇醱酵效果，具有工業化應用之潛力。

關鍵字

生質丁醇 ； 丁醇醱酵 ； 菌種鑑定

並列摘要

The study is the research of the biobutanol productivity by two anaerobic biobutanol producing strains isolated from soils. According to the analysis of 16S rDNA gene sequence alignment, the two strains were identified as Clostridium acetobutylicum, named Cl. acetobutylicum (S1) and Cl. acetobutylicum (S2). Both of them can be used to produce biobutanol by using glucose as carbon source. Cl. acetobutylicum S1 performed better effectiveness than Cl. acetobutylicum S2 in producing biobutanol. In the batch flask, Cl. acetobutylicum S1 utilized 60 g/L glucose and produced 7.5 g/L biobutanol, while the overall biobutanol productivity and yield were 0.25 g/L-h and 0.41 mol butanol/mol glucoe, respectively. The other products such as ethanol, acetone, butyric acid and lactic acid, were also produced in the process. The two strains had close productivity of the acetone: butanol: ethanol ratios (A: B: E ratio), were 2.76:22.7:1 (Cl. acetobutylicum S1) and 2.77:22.9:1 (Cl. acetobutylicum S2), respectively. Besides, Cl. acetobutylicum S1 could produce 14.2 g/L biobutanol and resulted in a higher yield (0.60 mol butanol/mol glucose) by fed-batch fermentation. The results showed that Cl. acetobutylicum S1 had the potential for industrial application.

並列關鍵字

Biobutanol ； Fermentative Production ； Identification of Strains