利用煤油分解菌進行甲基第三丁基醚生物降解之研究

Biodegradation of MTBE by Kerosene-Degrading Bacteria

林忠亮(Chung-Liang Lin) ； 方炳勳(Bing-Shiun Fang) ； 湯雅理(Ya-Li Tang) ； 林士民(Shyh-Mirn Lin)

《石油季刊》 48卷1期 (2012/03) Pp. 51-62

https://doi.org/10.30128/JP.201203.0005

摘要

本研究篩選出三株煤油利用菌，同時對MTBE具有良好的耐受度與降解能力。經菌種鑑定分別命名為Bacillus sp. M8801、Sphingomonas sp. M8802以及R. rubber M8803。其中R. rubber M8803對MTBE的降解能力最佳，對111mg/L MTBE最適降解條件為：30℃、pH 7.0下，於72小時內降解率達66%。R. rubber M8803對MTBE的降解屬好氧生物降解。在培養基中添加0.5 g/L酵母抽出物和0.5 g/L煤油可明顯提升R. rubber M8803對MTBE降解率達72%。上述結果顯示，可將之製備成微生物製劑，具有提供廢水處理現場以及土壤和地下水MTBE整治應用的潛力。

關鍵字

甲基第三丁基醚 ； 生物降解 ； R. rubber M8803

並列摘要

Three strains (designated as Bacillus sp. M8801, Sphingomonas sp. M8802, R. rubber M8803) of methyl tert-butyl ether (MTBE) degrading bacteria were isolated from oil-water interface of rain-sewage storage tank of petroleum refinery and an oil-contaminated site on located in southern Taiwan. In the MTBE biodegradation experiments, high efficiency of the MTBE degradation was found when the initial concentration of the MTBE was lower than 222 mg/L by R. rubber M8803. The optimal conditions for the biodegradation was found to be pH 7.0 and 30℃. The degradation rate of 66% for 111 mg/L MTBE could be achieved in 72 hours. Neither of R. rubber M8803 was able to grow and degrade MTBE anaerobically. Addition of yeast extract and 0.5 g/L kerosene to the medium had positive effect on R. rubber M8803 to degrade MTBE. The results showed that R. rubber M8803 has a very high potential for MTBE decontamination.

並列關鍵字

MTBE ； Biodegradation ； R. rubber M8803