THE EFFECTS OF GAMMA IRRADIATION STERILIZATION, TEMPERATURE, AND PH ON THE ANTIMICROBIAL ACTIVITY OF EPINECIDIN-1

Han-Ning Huang ； Chang-Jer Wu ； Jyh-Yih Chen

《Journal of Marine Science and Technology》 25卷3期 (2017/06) Pp. 352-357

https://doi.org/10.6119/JMST-016-1229-2

摘要

This study examined how different processing conditions affected the antibacterial activity of epinecidin-1 alone or in combination with deionized distilled water or KY jelly and evaluated its activity against methicillin resistant Staphylococcus aureus (MRSA) in a product development pipeline. High temperature and γ-irradiation (25 kGy) decreased antimicrobial activity of epinecidin-1, as determined by measuring minimal inhibitory concentrations (MICs) and minimal bactericidal concentrations (MBCs). However, epinecidin-1 exhibited high antimicrobial activity under low pH conditions. The pH stability and the wide spectrum of action against methicillin resistant Staphylococcus aureus (MRSA) may make epinecidin-1 peptide a suitable drug candidate. This research demonstrates the effects of gamma irradiation, temperature, and pH on the antimicrobial activity of epinecidin-1 in a product development pipeline. Its stability at low pH and wide spectrum of action against MRSA make the epinecidin-1 peptide an excellent drug candidate. Epinecidin-1 is effective at repressing the growth of MRSA, and it could be used as a lead for developing new antimicrobial agents against MRSA and related organisms.

關鍵字

gamma irradiation ； antimicrobial peptide ； epinecidin-1 ； methicillin-resistant Staphylococcus aureus (MRSA) ； antibacterial activity