This study aims at to investigate the influence of the introduction of stock future and option on margin trading of individual stocks. Using the underlying stocks of 234 stock futures and 43 stock options listed on Taiwan Futures Exchange, our findings show that there significantly exists the substitution and complementary effects of margin financing trading and margin short trading for the introduction of stock future and option. Additionally, by applying firm size, market-to-book ratio and turnover rate to separately capture the magnitude of substitution and complementary effects according to the substitution level of leverage trading and the enhancement of market efficiency, our results show the significant difference in substitution and complementary effects among the underlying stocks with different characteristics.