蛋白質缺乏誘導大鼠肝鐵堆積

Protein-Depletion Induces Hepatic Iron Accumulation in Rats

黃惠玲(Hui-Ling Huang) ； 蕭慧美(Huey-Mei Shaw) ； 蕭寧馨(Ning-Sing Shaw)

《嘉南學報（科技類）》 37期 (2011/12) Pp. 19-25

https://doi.org/10.29540/CNABST.201112.0004

摘要

本研究目的在於探討膳食蛋白質缺乏對於大鼠鐵代謝的影響。採用六週大S.D.雄鼠10隻，分成兩組：正常控制組（N組）與蛋白質缺乏組（0% protein，PF組），每組各5隻，飼養8天後犧牲，取血液與肝臟分析鐵代謝相關指標。結果顯示，PF組體重與血漿白蛋白顯著降低，血紅素與控制組相當，血球比容顯著高於C組，肝鐵濃度、鐵蛋白（ferritin）量、粒線體aconitase（m-ACO）酵素量分別為C組的1.8倍、9倍、1.7倍，肝鐵顯著堆積，且鐵儲存指標（ferritin）與功能性指標（m-ACO）均顯著增加；肝臟鐵調控蛋白IRPs之RNA結合活性，IRP1不受蛋白質缺乏的影響，IRP2於PF組顯著降低。結論：短期蛋白質缺乏，降低IRP2活性，顯著增加肝鐵濃度與鐵蛋白量，導致肝鐵堆積。

關鍵字

蛋白質缺乏 ； 鐵調控蛋白（IRPs） ； 鐵蛋白 ； 大鼠

並列摘要

The aim of this study was to investigate the effect of dietary protein depletion on the iron metabolism in rats. 10 male SD rats (6-wks-old) were assigned to 2 groups (n=5), normal control (N) and protein-free group (PF). After 8 d feeding, all animals were sacrificed by carbon dioxide asphyxiation. Blood and liver were collected for analysis of iron-associated parameters. Our results showed that PF group had significantly lower body weight and serum albumin compared with N group. Hb level had no difference between 2 groups, but Hct was higher in PF group. The hepatic iron, ferritin, and mACO in PF goup were 1.8, 9 and 1.7 fold, respectively. The IRP2 activity was significantly decreased in PF group. In summary, protein depletion showed a decrease in IRP2 activity and higher iron and ferritin contents in liver. There was significantly hepatic iron accumulation in protein-free group.

並列關鍵字

protein depletion ； IRPs ； ferritin ； rats