移動相中添加HP－β－環糊精在RPLC上分離二氯酚異構物

Separation of Isomeric Dichlorophenols by RPLC Using HP-β-cyclodextrin as Mobile Phase Additives

廖麗芳(Li-Fang Liao) ； 楊朝成(Chau-Chen Yang) ； 黃嘉慧(Chia-Hui Huang) ； 張書文(Shu-Wen Chang) ； 林振興(Chen-Hsing Lin)

《嘉南學報（科技類）》 38期 (2012/12) Pp. 55-60

https://doi.org/10.29540/CNABST.201212.0008

摘要

本研究在移動相中添加HP-β-CD，利用RP-C18靜相以分離6種二氯酚異構物。結果顯示在移動相中，HP－β－環糊精的濃度及甲醇與水比例，會顯著影響二氯酚異構物的分離效果。經由層析圖得知，添加1.25mM HP－β－環糊精於甲醇與水比例為50：50的混合溶液當移動相，利用RP-C18靜相管柱可以有效分離6種二氯酚異構物。從二氯酚異構物的滯留行為可知，包藏錯合物在分離機轉上扮演重要的角色。

關鍵字

逆相液相層析 ； HP－β－環糊精 ； 二氯酚

並列摘要

The isomeric dichlorophenols were separated by high-performance liquid chromatography using HP-β-cyclodextrin as mobile phase additives. Effects of HP-β-CD concentration and methanol content of mobile phase on the separation of isomeric dichlorophenols were investigated. Chromatogram of six isomeric dichlorophenols shows that the effective separations were achieved on a RPC18 stationary phase using 1.25mM HP-β-cyclodextrin as mobile phase additives. The chromatographic results indicate that inclusion complexes play a dominant role in the retention mechanism.

並列關鍵字

Reversed-Phase Liquid Chromatography (RPLC) ； HP-β-cyclodextrin ； dichlorophenol