台灣原生蕨類孢子微細結構之觀察與繁殖技術之研究

Studies on the Fine Structure of Spores and Multiplication Technique for the Native Ferns of Taiwan

陳進分(Jinn-Fen Chen)

《臺東區農業改良場研究彙報》 10輯 (1999/06) Pp. 1-14

https://doi.org/10.6959/RBTDAIS.199906.0001

摘要

採自台東區農業改良場蕨類種原圃之孢子，分成四種標本製作處理進行SEM觀察，處理A：採集新鮮孢子置於室內乾燥兩週，並貯藏六個月。處理B：新鮮孢子以冰醋酸進行孢子外皮分解，並經酒精脫水，空氣乾燥。處理C：新鮮孢子以2.5%戊二醛和0.05M磷酸緩衝液進行固定，並經酒精脫水，空氣乾燥。處理D：新鮮孢子不加處理。結果以新鮮孢子直接鍍膜觀察之處理最方便且效果最好。革葉鐵角蕨孢子繁殖試驗以蛇木屑為播種之介質最佳，播種季節以春夏最適，秋冬因低溫影響延遲發芽，且原葉體發育緩慢。播種溫度以日夜溫30/25℃最適。

關鍵字

蕨類 ； 孢子 ； 微細結構 ； 繁殖

並列摘要

Spores of this research were obtained from the greenhouse at Taitung DAIS. The methods of specimen preparation and observation on SEM were divided four treatments. Treatment A: The spores were only air dried for two weeks and stored for 6 months. Treatment B: Spores including sporangia were put in the tube. About 10 c.c. of room-temperature glacial acetic acid was added to the tube and kept in the boiling water bath for about 1 minute. Treatment C: Spores were placed in 2.5% glutaraldehyde +0.05M phosphate buffer (pH7.2), Samples were rinsed in 95% ethanol at 5 times and dehydrated in 95% ethanol. Treatment D: Fresh spores only.The best result is treatment D, fresh spores were mounted on standard stub and sputter coated with gold palladium for 1 minute.Tree fern debris is the best germination medium for Asplenium adiantoides propagation by using spore. The best time for A. adiantoides spore sowing are spring and summer in Taitung district because low winter temperature can affect the Prothallium growth.

並列關鍵字

Fern ； Spores ； Fine structure ； Multiplication