不同水稻幼苗萃取液抗氧化能力之測定

The Measure of Antioxidant Capacity of the Different Rice Seedlings' Extract

陳振義(Chen-I Chen)

《臺東區農業改良場研究彙報》 21輯 (2011/08) Pp. 27-36

https://doi.org/10.6959/RBTDAIS.201108.0027

摘要

本試驗利用臺東30號、臺稉9號、臺東糯31號、高雄秈糯8號、臺中秈10號及臺秈2號等6種栽培品種，在光照（綠化苗）及黑暗（黃化苗）兩種條件下，於發芽後7日及14日分別切取水稻幼苗地上部之莖菜，以乙醇及水萃取兩種方法，將上述材料製成萃取液，分別進行Trolox當量抗氧化能力測定、清除自由基能力測定、總酚類含量測定及還原力測定，比較不同幼苗萃取液的抗氧化能力。乙醇萃取法試驗結果，Trolox當量抗氧化測定以臺中秈10號14天黃化苗抗氧化能力最高；清除自由基能力以臺中秈10號14天綠化苗清除能力最高；總酚含量測定以臺中秈10號14天綠化苗含量最高，還原力測定結果，亦以臺中秈10號14天綠化苗還原力最高。以水萃取法試驗結果，Trolox當量抗氧化測定中以臺稉9號及臺中秈10號之14天的綠化苗抗氧化能力最高；清除自由基能力以臺中秈10號7天綠化苗清除能力最高；總酚含量以臺中秈10號14天綠化苗含量最高；還原力測定結果，亦以臺中秈10號14天綠化苗還原力最高。以上試驗結果可作為未來利用之參考。

關鍵字

水稻 ； 抗氧化能力 ； 綠化苗 ； 黃化苗

並列摘要

Antioxidant capacity, as measured by Trolox equivalent antioxidant capacity (TEAC), DPPH scavenging activity, total phenolic, and ferric reducing power were evaluated in the extraction of young seedling of six rice cultivars including Taitung 30, Taikeng 9, Taitung glutinous 31, Kaoshiung Sen glutinous 8, Taichung Sen 10, and Taisen 2. In ethanol fraction, 14-days etiolated seedling of Taichung Sen 10 had the highest values on the basis of TEAC assay. Values for DPPH scavenging, phenolics, and reducing powder in 14-days green seedling of Taichung Sen 10 were significantly higher than the other materials. In water soluble fraction, 14-days green seedling of Taikeng 9 and Taichung Sen 10 had much higher antioxidant activity based on TEAC assay than all other entities. Green seedling of 7-days old of Taichung Sen 10 had the highest DPPH scavenging ability. Phenolic content and reducing power were higher than the others in 14-days green seedling of Taichung Sen 10. These results could be useful for future application.

並列關鍵字

Rice ； Antioxidant capacity ； Green seedlings ； Etiolated seedlings