Dual Involvement and Its Potential Significance of Epstein-Barr Virus and Human T Lymphotropic Virus Type I in Nasopharyngeal Carcinoma

It has been increasingly evident that multiple factors are involved in the cause of cancer. We demonstrated previously, DNA sequences related to human T lymphotropic virus type I (HTLV-1) in leukemia and lymphoma as well as carcinoma of certain types in man, and showed that these sequences caused genetic instability of host cells. This study was carried out to examine the possible involvement of HTLV-1 in nasopharyngeal carcinomas (NPC) in which Epstein-Barr virus (EBV) is etiologically implicated, and to discuss potential significance of the involvement. DNA extracted from tumor tissues of 18 patients with NPC was examined for the presence of DNA coding for EBNA-1, LMP-1, HTLV-1-LTR, -gag, and -tax, by PCR-SSCP or PCR-PAGE. EBER-1 and HTLV-I-LPR were examined by in situ hybridization. Serial sections were tested by immunostaining with monoclonal antibody for LMP-1, Ki-67, PCNA, Rb, p53, HER-2/neu, bcl-2, Fas, and BM-1. Positive reactions were seen in 13, 9, 18, 18, 0, 17, 14, 15, 5, 16, 10, 9, and 10 specimens, for EBNA-1, EBER-1, LMP-1, HTLV-I, LMP-1 protein, Ki-67, PCNA, Rb, p53, HER-2/neu, bcl-2, Fas, and BM-1, respectively. Direct sequencing from PCR products of representative cases revealed multiple mutations in HTLV-1-LTR and in bcl-2 sequences analyzed. These results demonstrated the dual involvement of EBY and HTLV-1 in NPC, and deregulation of cellular genes of host cells.

並列關鍵字

NPC ； EBV ； HTLY-1 ； Deregulated gene product expression

被引用紀錄

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鄭永強（2015）。新型磁流變液式可變氣門正時與升程機構之設計與動態分析〔碩士論文，國立虎尾科技大學〕。華藝線上圖書館。https://doi.org/10.6827/NFU.2015.00192

杜政憲（2011）。新型雙搖臂式汽門機構的設計與動態模擬分析〔碩士論文，國立虎尾科技大學〕。華藝線上圖書館。https://doi.org/10.6827/NFU.2011.00013

陳裕智（2010）。使用ADAMS在新型雙搖臂式無段可變正時與升程汽門機構動態模擬分析〔碩士論文，國立虎尾科技大學〕。華藝線上圖書館。https://www.airitilibrary.com/Article/Detail?DocID=U0028-0206201000423500

延伸閱讀

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國際替代計量

Dual Involvement and Its Potential Significance of Epstein-Barr Virus and Human T Lymphotropic Virus Type I in Nasopharyngeal Carcinoma

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