- 期刊
維生素C對於KB及CHO細胞放射線敏感度之影響
Effects of Vitamin C on the Radiosensitivity of KB and CHO Cells
摘要
目的：本研究之目的在探討維生素C(vitamin C, ascorbic acid, AA)對於口腔癌(KB)及中國倉鼠卵巢(CHO)細胞放射線敏感度之影響。 材料與方法：本實驗將細胞共分為三組：A組為正常對照組，B組為AA給與後立即(0小時)接受放射線照射，C組為AA處理後24及48小時再接受放射線照射。 結果；研究發現AA對於KB及CHO細胞之生長有抑制作用。AA對於KB及CHO細胞之ID50值分別為2.48及2.11 mg/ml（培養液），兩者非常接近。在KB細胞培養中加入AA後0,24及48小時，分別給予單一放射劑量800 cGy照射，可發現KB細胞之生存分數(survival fraction, SF)隨著時間增加而增加，且CHO細胞SF之變化也有相同趨勢。一般說來培養後愈久時間，接受放射線照射之SF值也愈高。但若分別與B組細胞之SF值相比，AA加入後24及48小時再分別接受放射線照射之KB細胞其SF值增加倍數無顯著變化。顯示AA對於KB細胞沒有明顯的輻射保護作用。相反的，與B組細胞之SF值相比，CHO細胞之SF增加倍數有極顯著的增加，表示AA對CHO細胞有明顯的輻射保護作用。結論：當CHO細胞培養液內加入AA後，在培養後48小時內，隨著時間的增加AA能提供之輻射保護作用也愈明顯。此種輻射保護作用在KB細胞並不顯著，在CHO細胞則非常明顯。而此種差別性的保護正常細胞可能有助能AA在放射治療上的應用。
並列摘要
Purpose: The goal of this study is to realize the radioprotective effects of vitamin C (ascorbic acid, AA) on KB and CHO cells. Materials and Methods: The experimental cells were assigned to divide into three groups: group A was the normal control; Cells in group C were irradiated at 24 or 48 hrs respectively after AA treatment. Results: There is an inhibitory effect of AA on the growth of KB and CHO cells. The ID50 data of AA on KB and CHO cells are 2.48 and 2.11 mg/ml of culture medium respectively, roughly the same of them. The survival fractions (SF) of KB cells increased gradually after delivered AA to their culture medium at 0, 24 and 48 hrs, and then irradiated with a single fraction of 800 cGy. Similar results were also noted in CHO cells. In general, the longer of the culture period, the higher of the SF after irradiation at that culture time. Compared to the SF of group B (0 hr), there was no significant increase of SF at 24 and 48 hrs in CHO treated KB cells. It showed that there was no radioprotective effect of AA on KB cells. On the contrary, the SF of CHO cells treated with AA at 24 and 48 hrs respectively increased significantly compared to the SF of group B. Conclusion: The radioprotective effects of AA on CHO cell were proportional to culture time within 48 hrs after AA treatment. This radioprotective effect was not significant in KB cells, but was significant in CHO cells. The differential radioprotective effect of AA on normal cells may be used in clinical radiotherapy.
延伸閱讀
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