以人類肝癌細胞測試硼碘化油在中子捕獲治療上之生物效應

Biological Efficacy of B-Lipiodol for Neutron Capture Therapy as Mesasured in Human Hepatoma Cell Culture

周鳳英(F. I. Chou) ； 衛元耀(Y. Y. Wei) ； 蔣淑娟(S. J. Jeang) ； 王天戈(T. K. Wang) ； 開執中(J. J. Kai) ； 戚謹文(C. W. Chi) ； 雷永耀(W. Y. Lui)

《放射治療與腫瘤學》 3卷4期 (1996/12) Pp. 215-220

https://doi.org/10.6316/TRO/199603(4)215

摘要

目的：本研究之目的為研製含硼碘化油(B-lipiodol)，探討人類肝癌細胞(HepG2 Cells)對B-lipiodol之積聚效果及中子照射後之細胞毒性。
材料與方法：培養中之人類肝癌細胞測試其對B-lipiodol之攝入及積聚。以光學顯微鏡檢視B-lipiodol與肝癌細胞間之交互作用。以感應偶合電漿原子發射光譜分析儀分析硼含量。經B-lipiodol處理之肝癌細胞於清華水池式反應器中接受中子照射。
結果：血清中穩定性測試，結果顯示硼可穩定存於B-lipiodol中。肝癌細胞可大量攝入、積聚B-lipiodol。在我們的測試條件下，中子捕獲對B-lipiodol處理之肝癌細胞具有極高之細胞毒性、劑量反應曲綫相當陡。
結論：受測人類肝癌細胞會攝入B-lipiodol，且有足够量之硼會滯留於細胞中以供中子捕獲反應用。B-lipiodol應用於人類肝癌細胞之硼中子捕獲殺傷上是一具高細胞毒性藥物。

關鍵字

硼碘化油 ； 肝癌細胞 ； 硼中子捕獲治療

並列摘要

Objective: The objective of this study was designed to preparation of the boron containing lipiodol (B-lipiodol) for boron neutron capture therapy, and evaluation of the cytotoxicity of B-lipiodol on hepatoma cells after neutron irradiation.
Materials and Methods: HepG2 cell culture was used to examine of the uptake and retention of B-lipiodol. Light microscopes were used for examination of the interaction and retention of 8-lipiodol droplets in the individual cell. Boron concentration was determined by ICPASS analysis. THOR was used for irradiation of the B-lipiodol treated HepG2 cells.
Results: Stability test showed that boron was stably retained in lipiodol contrast medium. HepG2 cells appeared proficiently at internalization and retention of B-lipiodol. Under our tested condition, boron neutron capture was highly cytotoxic in B-lipiodol treated HepG2 cells. There was a steep dose response relationship.
Conclusion: Hepatoma cells are capable of active uptake of B-lipiodol and enough amount of boron was retention inside the cells for neutron capture reaction. B-lipiodol is a highly cytotoxic and effective drug for the neutron-capture killing of FIepG2 cells.

並列關鍵字

B-lipiodol ； Hepatoma cell ； Boron neutron capture therapy