乳酸桿菌科菌株提高免疫力及抗癌方面的研究

The Study of Immunopotentiating and Anti-Tumor Abilities of Lactobacteriaceae

羅敏菁(Ming-Ching Lo) ； 季匡華(Kwan-Hwa Chi)

《放射治療與腫瘤學》 5卷2期 (1998/06) Pp. 111-117

https://doi.org/10.6316/TRO/199805(2)111

摘要

目的：測試乳酸桿菌科菌株提高免疫力之效果及其抗癌方面的表現。
材料與方法：本研究以不同來源之乳酸捍菌科死菌體及靈芝萃取液，能否在體外刺激老鼠脾臟細胞產生γ－擾素，做為其增強免疫力的指標；再以遲發性過敏反應(Delayed Type Hypersensitlvity; DTH) 測試乳酸捍菌科菌株的佐劑作用及其引發的T細胞記憶反應；最後以同時注射方式，測定乳酸桿菌科死菌體抑制肉瘤細胞(CT-26)的生長情形，並與BCG比較之。
結果：對體外培養的老鼠脾臟細胞，所有的乳酸桿菌科死菌體在刺激γ－擾素的生成作用上都比靈芝強，而比Con A稍差。同時施打羊紅血球(SRBC)和乳酸捍菌科死菌體時，可以稍微增加皮膚的腫脹程度，表示它對遲發性過敏反應有輕度的佐劑作用。而先打死菌體刺激局部的免疫系統，再以SRBC免疫時，局部的遲襲性過敏反應要比未打死菌體的對照組強。另外同時注射死菌體與腫瘤細胞時，死菌體比BCG活菌更能抑制肉瘤細胞的生長。
結論：經本實驗獲得初步的證實，乳酸桿菌科菌株可增強免疫反應並抑制癌細胞的生長，但其完整的作用機轉，還須進一步深入研究。

關鍵字

乳酸桿菌科 ； 免疫增強作用 ； 抗癌 ； 遲發性過敏反應 ； 佐劑

並列摘要

Purpose: To examine the immunopotentiating ability of Lactobacteriaceae with Ganoderma lucidum in vitro and compare the anti-tumor ability of Lactobactcriaceac with that of BCG using a mouse model injected with sarcoma cells.
Materials and Methods: The immunopotentiating ability was tested in vitro using the stimulation of interferon- gamma production by mouse spleen cells. The delayed type hypersensitivity shown by foot swelling scale was chosen as an index of adjuvant effect. Anti-tumor ability was examined by simultaneously injected bacteria cells with sarcoma cells on mouse left hind leg.
Results: The concentrations of interferon-gamma proliferation by various heat-killed Lactobateriaceae cells were higher than four tested Ganoderma lucidum products, but slightly lower than Con A. Simultaneously injected SRBC with heat-killed Lactobacteriaceae cells showed an adjuvant effect on foot swelling in mouse model. Mice immunized with heat-killed Lactobacteiraceae cells had higher foot swelling scale when challenged with SRBC three weeks later. Heat-killed Lactobateriaceae cells demonstrated a better inhibition of tumor growth than viable BCG.
Conclusion: The immunopotentiating and anti-tumor abilities of Lactobacteriaceae are initially verified, further studies should be carried on.

並列關鍵字

Lactobacteriaceae ； Immunopotentiation ； Anti-tumor ； Delayed type hypersensitivity ； Adjnvant