The Combined Effect of Colchicine and Radiation of Human Hepatoma HA22T/VGH Cells

秋水仙素結合放射線對人類HA22T/VGH肝癌細胞之作用

陳裕仁(Yu-Jen Chen) ； 徐世達(Shyh-Dar Shyur) ； 張愛珠(Anita Chang) ； 陳弘政(Hung-Cheng Chen) ； 張國華(Kou-Hwa Chang) ； 賴允亮(Yuen-Liang Lai) ； 陳姵君(Pai-Gene Chen)

《放射治療與腫瘤學》 6卷2期 (1999/06) Pp. 83-87

https://doi.org/10.6316/TRO/199906(2)83

摘要

目的：本研究旨在測試其是否可降低人類HA22T/VGH肝癌細胞接受放射線後之存活率。材料與方法：HA22T/VGH細胞經秋水仙素處理24小時後，接受不同劑量放射線，75小時後用MTT方法測試細胞活度；並計數照射前的有絲分裂百分比及觀察其形態變化。
結果：秋水仙素在極低劑量下(8ng/ml)即可降低人類HA22T/VGH肝癌細胞接受放射線後之存活率；伴隨此現象可見到秋水仙素增加細胞的有絲分裂百分比。另外，HA22T/VGH細胞經秋水仙素處理後，在形態上變成圓形、浮起且呈現明顯的分裂停滯現象。
結論：上述結果顯示秋水仙素可降低人類HA22T/VGH肝癌細胞接受放射線後之存活率。

關鍵字

放射線 ； 秋水仙素 ； 肝癌細胞

並列摘要

Purpose: We studied colchicine to determine whether or not it can reduce the survival of irradiated human hepatoma HA22T/VGH cells.
Materials and Methods: Twenty four hours after treatment of 0-8ng/ml colchicine, HA22T/VGH cells were irradiated with various doses. Seventy five hours later, the MTT[(3-(4,5-dimethylthiazol-2-y-2,5-diphenyl tetrazolium bromide)] assay was performed to assess cell viability. Before radiation, the percentage of mitosis was calculated and micrographs were taken for morphological observation.
Results: Colohicine at a low concentration of 8ng/ml enhanced the growth-inhibiting effect of radiation (up to 10-folds compared to 2Gy radiation alone). We also noted a parallel effect of colchicine on increasing the percentage of mitosis of cells before radiation. Cells treated with colchicine became rounded, floated up and showed mitotic features under observation by an inverted light microscope.
Conclusion: These findings suggest that colchicine has potential as an adjunct to radiotherapy for hepatoma.

並列關鍵字

Radiation ； Colchicine ； Hepatoma HA22T/VGH cell