放射線照射及BSO處理後多形性神經膠母瘤細胞內麩氨基硫含量變化之研究

Effects of Radiation and BSO on GSH Concetration of GBM Cells

黃經民(Jing-Min Hwang) ； 黃旭山(Hsu-Shan Huang) ； 許文林(Wen-Lin Hsu) ； 熊佩韋(Pei-Wei Shueng) ； 李偉華(Wei-Hwa Lee)

《放射治療與腫瘤學》 7卷1期 (2000/03) Pp. 1-8

https://doi.org/10.6316/TRO/200007(1)1

摘要

目的：分析放射線照射及BSO處理對於多形性神經膠母瘤細胞(glioblastoma multiforme, GSM)內GSH含量之影響。
材料與方法：本實驗採用GBM細胞株為實驗對象，首先進行GBM細胞株內麩基硫(glutathione，GSH)含量之測定，接著以Lowrymethod測定細胞內蛋白質之濃度，然後以放射線照射及/或BSO(D，L-buthionine-S，R-sulfoximine)處理細胞，研究在有氧及缺氧環境下，不同劑量放射線照射及/或BSO處理後細胞GSH含量改變之情形。
結果：GBM細胞內GSH之濃度([GSH])約為9.93±0.46(ug/mg of cellular protein，以下單位相同故省略)，經過2-14Gy之放射線照射後不同時間，隨著放射劑量的增加[GSH]有上升的趨勢。細胞經BSO處頭龍之濃度矯8.78±0.05，經BSO處理及放射線照射後不同時間之[GSH]隨著放射線劑量之增加也有上升的趨勢。缺氧GBM細胞內[GSH]約為32.45±0.25，經BSO處理後之濃度為13.36±0.47，經放射線照射/或BSO處理及放射線照射後不同時間之[GSH]隨著照射劑量之增加多有升高的現象。有氧之GBM細胞經BSO處理後細胞內[GSH]有下降之情形，但是缺氧GSM細胞只有在照射後立即至分析這一組[GSH]減少，經過24及48小時再分析之兩組[GSH]無明顯變化。除了在有氧狀態照射後立即分析組，其餘組之細胞經放射線照射後，隨著放射劑量的增加[GSH]有上升的趨勢，且此上升趨勢經過BSO處理後更為明顯。此外，放射線照射後之GBM細胞內(GSH)變化具有氧或缺氧狀態無顯著關係。
結論：（一）有氧GBM細胞經BSO處理後細胞內[GSH]有下降之情形，但是缺氧GBM細胞只有在照射後立即分析這一組[GSH]減少。仁)放射線照射後之GSM細胞內(GSH)變化具與有氧或缺氧狀態無顯著關係。一般而言，GBM細胞經放射線照射後，隨著放射劑量的增加[GSH]有益漸上升的趨勢，且此上升趨勢經BSO處理或在照射後24~48小時更為明顯。此種[GSH]逐漸上升可能表示隨著放射劑量增加細胞修復愈不明顯且輻射生物鼓應亦愈加顯著。

關鍵字

麩氨基硫,多形性神經膠母瘤,放射治療

並列摘要

Purpose: To evaluate the efficacy of radiation and BSO on the GSH concentration of GBM cells.
Materials and Methods : Exponentially growing cultures of GBM/8401 cells were used for this study. The GSH concentrations in GBM cells treated with radiation and/or BSO (D, L-buthionine-S, R-sulfox-imine) were determined. Cells were irradiated with dosage ranged from 0 to 14 Gy with gamma radiation using a cobalt 60 machine. The concentration of GSH in irradiated GBM cells treated with or without BSO was evaluated under aerobic and hypoxic conditions.
Result: The intracellular concentration of glutathione ([GSH]) was 9.93± 0.46 ug/mg of cellular protein. In general, the [GSH] increased gradually after irradiated by cobalt-60 with 2~14 Gy. The [GSH] in OBM cells were 8.78±0.05 after treated with BSO. Also, [GSH] increased gradually after irradiated from low to high dose of irradiation. The [GSH] in hypoxic GBM cells were 32.45±0.25. It was 13.36±0.47 after treated with BSO. In general, the cellular [GSH] after irradiated from low to high dose of radiation increased gradually no matter treated with BSO or not. BSO is able to deplete the intracellular [GSH] but this is not significant in hypoxic cells 24 and 48 hours after treatment. Change of intracellular [GSH] is also not closely related with the present of oxygen or not.
Conclusion: (1). In aerobic group, the GSH concentrations in OBM cells decrease after treated with BSO. In hypoxic group, the GSH concentration decreased only in cells analyzed immediately after irradiation. (2). There is no significant association between GSH concentrations of irradiated GBM cells and various oxygenated conditions. (3). In general, GSH concentration increase in proportional to radiation dose. This is more evident after treated the cells with BSO or 24~48 hours after irradiation Gradually increased concentration of GSH represents less repair of the damaged cells from radiation and more biologic effects in accordance with high radiation dose.

並列關鍵字

Glutathione ([GSH]),Glioblastoma muItiforme (GBM),BSO (D, L-buthionine-S, R-sulfoximine),Radiation