耐力運動訓練對誘發骨骼肌細胞凋亡之粒腺體訊息傳遞路徑的影響

The Effect of Endurance Exercise Training on the Mitochondria Signaling Pathway of Apoptosis in Skeletal Muscle

陳聰毅(Tsung-I Chen) ； 林念璁(Wu-Chu Chen)

《體育學報》 39卷4期 (2006/12) Pp. 11-22

https://doi.org/10.6222/pej.3904.200612.1402

摘要

Purpose: This study is to investigate the influence of exercise training on skeletal muscle apoptosis induced by mitochondrial pathway. Methods: Animals, male Wistar rats, were randomly assigned to either a control group or an exercise group. Exercise group were trained for 5 days weekly for 12 weeks on treadmill. An exhausted exercise test was performed after a rest period of 7 days after 12 weeks exercise training. Rats were sacrificed at pre-exercise (PRE), post-exercise 0 hour (PEO) and post-exercise 48 hour (PE48), respectively. The soleus and gastrocnemius were dissected out and frozen in liquid nitrogen rapidly. Results: The citrate synthesis activity of soleus of trained was significantly higher than untrained at PRE, PE0 and PE48 (p＜.05), respectively. Both the muscle weight-to-body weight ratio of gastrocnemius and soleus of trained were significantly higher than untrained (p＜.05). In Bcl-2 expression, both of the gastrocnemius and soleus of trained were significantly higher than untrained at PE0 (p＜.05) and significantly lower than untrained at PE48 (p＜.05). In Bax expression, both of the gastrocnemius and soleus of trained were significantly lower than untrained at PE0 and PE48 (p＜.05), respectively. Both the apoptosis index of gastrocnemius and soleus of trained were significantly lower than untrained after PE48 (p＜.05). Conclusion: These findings suggest that skeletal muscle may modulate their Bcl-2 and Bax apoptotic signals expression and the oxidative activity of mitochondria after exercise training, lead to attenuating the time of skeletal muscle apoptosis or inhibiting the apoptotic signals expression, and then the probability of hypertrophy occurred.

關鍵字

細胞凋亡 ； 粒腺體 ； 運動訓練 ； 骨骼肌

並列摘要

Purpose: This study is to investigate the influence of exercise training on skeletal muscle apoptosis induced by mitochondrial pathway. Methods: Animals, male Wistar rats, were randomly assigned to either a control group or an exercise group. Exercise group were trained for 5 days weekly for 12 weeks on treadmill. An exhausted exercise test was performed after a rest period of 7 days after 12 weeks exercise training. Rats were sacrificed at pre-exercise (PRE), post-exercise 0 hour (PEO) and post-exercise 48 hour (PE48), respectively. The soleus and gastrocnemius were dissected out and frozen in liquid nitrogen rapidly. Results: The citrate synthesis activity of soleus of trained was significantly higher than untrained at PRE, PE0 and PE48 (p＜.05), respectively. Both the muscle weight-to-body weight ratio of gastrocnemius and soleus of trained were significantly higher than untrained (p＜.05). In Bcl-2 expression, both of the gastrocnemius and soleus of trained were significantly higher than untrained at PE0 (p＜.05) and significantly lower than untrained at PE48 (p＜.05). In Bax expression, both of the gastrocnemius and soleus of trained were significantly lower than untrained at PE0 and PE48 (p＜.05), respectively. Both the apoptosis index of gastrocnemius and soleus of trained were significantly lower than untrained after PE48 (p＜.05). Conclusion: These findings suggest that skeletal muscle may modulate their Bcl-2 and Bax apoptotic signals expression and the oxidative activity of mitochondria after exercise training, lead to attenuating the time of skeletal muscle apoptosis or inhibiting the apoptotic signals expression, and then the probability of hypertrophy occurred.

並列關鍵字

apoptosis ； mitochondrial ； exercise ； skeletal muscle

參考文獻

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