利用串聯式液相層析質譜法同時分析丙烯醯胺職業暴露工人尿液代謝物

Simultaneous Determination of Urinary Mercapturic Acids for Acrylamide Exposed Workers by LC-MS/MS

吳焜裕(Kuen-Yuh Wu) ； 陳美蓮(Mei-Lien Chen) ； 劉紹興(Saou-Hsing Liou) ； 汪禧年(Shi-Nian Uang) ； 黃鈺芳(Yu-Fang Huang)

《勞工安全衛生研究季刊》 17卷3期 (2009/09) Pp. 385-397

https://doi.org/10.7005/JOSH.200909.0385

摘要

Acrylamide (AA) has been widely used in industry to produce flocculants and grouting agents as well as in biological laboratories for the preparation of polyacrylamide gels for electrophoresis. It is classified as a probable human carcinogen (Group 2A) by the IARC. Workers are at risk of AA exposures. AA can be metabolized into AA N-acetyl-S-(propionamide)-cysteine (AAMA) and of Glycidamide (GA) N-acetyl-S-(carbamoyl-2-hydroxyethyl)-cysteine (GAMA2) and N-acetyl-S-(3- amino-2-hydroxy-3-oxopropyl)-cysteine (GAMA3). Analysis of these mercapturic acids (MAs) can serve as biomarkers for AA exposures and illustrate the metabolism of AA in human. In our study, a liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS) method was developed to simultaneously analyze urinary AAMA, GAMA2 and GAMA3 for the AA-exposed workers. Fifty six AA-exposed workers and 36 controls were recruited and provided a pair of pre- and post-shift urine samples for this study.
The results showed the limit of detection (LOD) values were ＜10 ng/mL (GAMA2) , 20 ng/mL (GAMA3) and 5 ng/mL (AAMA) in urine and the detection rate was 34%, 38% and 92% for GAMA2, GAMA3 and AAMA, respectively. The geometric mean (range) level of GAMA2, GAMA3 and AAMA in the whole collective (n=100) was 38.2 (8.1-343) (μg/g creatinine), 94.9(10.8-1754.6) (μg/g creatinine) and 516.7 (4.7-115843) (μg/g creatinine), respectively. There was a significantly positive correlation between naturally log-transformed concentrations of AAMA and naturally log-transformed GAMA2 and GAMA3 (Pearson correlation: r=0.695, p＜0.001). Besides, the pre- and post-shift urinary AAMA concentrations for the AA-exposed workers were higher than those for the controls. These results suggest that AAMA can serve as a sensitive, specific, non-invasive, and easily accessible biomarker for AA exposures.

關鍵字

丙烯醯胺 ； 尿液代謝物N-acetyl-S-propionamide-cysteine AAMA ； N-acetyl-S-carbamoyl-2-hydroxyethyl-cysteine GAMA2 ； N-acetyl-S-3-amino-2-hydroxy-3-oxopropyl-cysteine GAMA3 ； 液相層析串聯式質譜儀

並列摘要

Acrylamide (AA) has been widely used in industry to produce flocculants and grouting agents as well as in biological laboratories for the preparation of polyacrylamide gels for electrophoresis. It is classified as a probable human carcinogen (Group 2A) by the IARC. Workers are at risk of AA exposures. AA can be metabolized into AA N-acetyl-S-(propionamide)-cysteine (AAMA) and of Glycidamide (GA) N-acetyl-S-(carbamoyl-2-hydroxyethyl)-cysteine (GAMA2) and N-acetyl-S-(3- amino-2-hydroxy-3-oxopropyl)-cysteine (GAMA3). Analysis of these mercapturic acids (MAs) can serve as biomarkers for AA exposures and illustrate the metabolism of AA in human. In our study, a liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS) method was developed to simultaneously analyze urinary AAMA, GAMA2 and GAMA3 for the AA-exposed workers. Fifty six AA-exposed workers and 36 controls were recruited and provided a pair of pre- and post-shift urine samples for this study.
The results showed the limit of detection (LOD) values were ＜10 ng/mL (GAMA2) , 20 ng/mL (GAMA3) and 5 ng/mL (AAMA) in urine and the detection rate was 34%, 38% and 92% for GAMA2, GAMA3 and AAMA, respectively. The geometric mean (range) level of GAMA2, GAMA3 and AAMA in the whole collective (n=100) was 38.2 (8.1-343) (μg/g creatinine), 94.9(10.8-1754.6) (μg/g creatinine) and 516.7 (4.7-115843) (μg/g creatinine), respectively. There was a significantly positive correlation between naturally log-transformed concentrations of AAMA and naturally log-transformed GAMA2 and GAMA3 (Pearson correlation: r=0.695, p＜0.001). Besides, the pre- and post-shift urinary AAMA concentrations for the AA-exposed workers were higher than those for the controls. These results suggest that AAMA can serve as a sensitive, specific, non-invasive, and easily accessible biomarker for AA exposures.

並列關鍵字

Acrylamide ； N-acetyl-S-propionamide-cysteine AAMA ； N-acetyl-S-carbamoyl-2-hydroxyethyl-cysteine GAMA2 ； N-acetyl-S-3-amino-2-hydroxy-3-oxopropyl-cysteine GAMA3 ； Liquid chromatography-tandem mass spectrometry LC-MS/MS

參考文獻

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