This research is to investigate the comfort of human body by utilizing moisture-transferring and quick-dried fabrics and cotton fabrics. With the advantages of giving off human body quickly of moisture-transferring and quick-dried fabrics, the values of the heart rate variability and body's surface temperatures are obtained in static and dynamic state to analyze the comfort of human body. In this research, with the comparison between sympathetic and parasympathetic nerves, we realize that the fabrics with the treatment of the hydrophilic finishing have better comfort than other moisture-transferring and quick-dried fabrics.