低溫保存對台灣紅豆杉莖芽存活、生長與紫杉烷類含量的影響

台灣紅豆杉莖芽以4及12℃低溫保存3 mo，再恢復於正常培養室培養1.5 mo以探討莖芽長度、繼代培養後莖芽置入低溫前之預培養時間、培養基組成與光照條件對莖芽存活的影響。結果以1.5cm之莖芽，在正常培養室預培養2wk，培養基為MS添加10μMABA的低溫保存芽體存活率最高。該處理在不同低溫、黑暗或低光照的環境，均可獲得97%以上的存活率。延長莖芽在12℃低溫保存時間到0.5，1，1.5與2yr後的莖芽存活率分別為97，90，87與72%；在4℃則為95，75，72，59%。低溫保存後存活之莖芽雖然有褐化與壞死現象，但仍能會恢復正常生長。比較低溫保存2yr之莖芽與正常繼代培養莖芽生產紫杉烷類之濃度，發現兩者沒有差異。建議最佳低溫保存方式是12℃黑暗下1.5yr，此條件下莖芽可以達到87%的存活率。

關鍵字

台灣紅豆杉；莖芽；低溫保存；紫杉烷類化合物；紫杉醇

並列摘要

Taxus mairei (Lemee & Levl.) SY Hu ex Liu shoots were stored in vitro at 4 and 12℃ for 3 mo. They were then recultured with normal culture conditions for 1.5 mo to determine the factors which affected their survival, such as shoot size, preculture period prior to storage at low temperature, medium composition, and illumination. Shoots transferred into normal conditions for 1.5 mo after low-temperature storage, which were 1.5 cm long and precultured on MS medium containing 10 μM ABA for 2 wk, exhibited the greatest survival rates compared to the other treatments. In this pretreatment, the survival rates of shoots stored at 2 low temperatures and cultured in either light or dark exceeded 97%. When extending the period of cold storage from 3 to 24 mo, survival rates of cultures after being stored for 0.5, 1, 1.5, and 2 yr were 97, 90, 87, and 72% respectively at 12℃, and 95, 75, 72, and 59% respectively at 4℃. Although leaf browning and necrosis occurred during the low-temperature storage, the growth of surviving shoots recovered well. The concentrations of taxanes between regularly subcultured shoots and 2-yr-old shoots placed in cold storage did not significant differ This suggests that the best way for long-term storage is to store the in vitro shoots in the dark at 12℃.

並列關鍵字

Taxus mairei ； shoots ； cold storage ； taxane ； taxol

國際替代計量

低溫保存對台灣紅豆杉莖芽存活、生長與紫杉烷類含量的影響

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