- 期刊
- OpenAccess
Micropropagation of 〞Quercus aliena Blume var. aliena〞 from Explants of Mature Trees
以成熟林木培殖體進行槲櫟微體繁殖
摘要
本研究建立槲櫟（Quercus aliena Blume var. aliena）成熟林木組織培養微體繁殖之流程，採用並修改在同屬樹種中已經成功應用之培養條件，測試其在槲櫟之適用效果。微體繁殖的步驟以收集樹幹基部萌芽之節莖為培殖體，切取新誘導形成之側芽（長度≥1.5cm）、去葉及截頂後橫放培養於GD_1培養基中並添加0.88μM 6-benzylaminopurine（BAP）及不同濃度的硝酸銀，以8週為一培養週期並逐次遞減BAP濃度，可促進側芽的誘導及增殖。試驗中截頂之培殖體水平橫放培養及添加17.67μM的硝酸銀可促進芽體增殖及抽長，若添加活性碳則否。抽長芽體利用123.0μM indole-3-butyric acid（IBA）進行48h的發根誘導，接著將誘導後之芽體培養在不含IBA，但添加17.67μM硝酸銀的培養基中，有較佳發根率（87.5%）。發根芽體移盆健化時存活率為72.3%，並有多數小植株已能在溫室中正常生長。
並列摘要
This study established a micropropagation method for the tissue culture propagation of mature 〞Quercus aliena Blume var. aliena.〞 The method was modified from procedures successfully applied to other species of the same genus, and it was tested for its efficiency on mature explants of the target species. Stem nodal segments collected from trunk sprouts were used as the original explants. The newly induced axillary shoots (≥ 1.5cm long) from the explants were defoliated, decapitated, and horizontally cultured on GD_1 medium supplemented with 0.88μM 6-benzylaminopurine (BAP) and various concentrations of AgNO_3. Axillary shoot proliferation was obtained through an 8-wk cyclic culture in which the concentration of BAP gradually decreased. Decapitation and horizontal culture of explants together with the addition of 17.67 μM AgNO_3 proved beneficial for shoot proliferation and elongation; however, the efficiency of this method diminished when activated charcoal was added. The elongated shoots were rooted with indole-3-butyric acid (IBA, 123μM) pulse stimulation for 48h, after which the shoots were transferred to IBA-free root expression medium with 17.67 μM AgNO_3, and they achieved a better rooting percentage (87.5%). Rooted plantlets were acclimatized and successfully grown in a greenhouse, with a survival rate of 72.3%.
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