印度塊菌、麗江塊菌及深脈塊菌菌絲培養之研究

Study of the in vitro Culture of Tuber indicum, T. lijiangense and T. elevatireticulatum Mycelium

傅春旭(Chuen-Hsu Fu) ； 蔡明哲(Ming-Jer Tsai) ； 李鎧彤(Hoi-Tung Li) ； 黃勁暉(King-Fai Wong) ； 張詠怡(Wing-Yi Cheung)

《台灣林業科學》 34卷2期 (2019/06) Pp. 127-134

https://doi.org/10.7075/TJFS.201906_34(2).0005

摘要

將印度塊菌（Tuber indicum）、麗江塊菌（T. lijiangense）及深脈塊菌（T. elevatireticulatum）於1/2 MS上作菌種分離，進行分子生物鑑定，與基因庫的基因序列作比對，確定所分離出的菌株分別亦是印度塊菌、麗江塊菌及深脈塊菌。將印度塊菌菌株次培養到不同的培養基，發現其菌株可在1/2 MS、YEA、PDA、YMTA、Soil extract培養基上生長；印度塊菌及深脈塊菌在1/2 MS培養基中有較快的生長；麗江塊菌在YMTA培養基中有較快的生長。展望掌握塊菌的菌種分離及菌絲培養，可以進一步確立塊菌與不同樹種的共生關係外，亦期望可以應用至塊菌產業生產中。

關鍵字

塊菌 ； 菌株分離 ； 分子生物鑑定 ； 顯微特徵 ； 培養基篩選

並列摘要

Pure cultures of Tuber indicum, T. lijiangense, and T. elevatireticulatum were obtained through isolation of fertile tissues of fruiting bodies in 1/2 Murashige and Skoog (MS) agar medium. Molecular identification of the fruiting bodies and pure cultures were carried out. Tuber indicum was subcultured on different types of agar medium and showed growth on 1/2 MS, yeast extract agar (YEA), potato dextrose agar (PDA), YMTA, and soil extract agar medium. Tuber indicum and T. elevatireticulatum exhibited the best growth on 1/2 MS medium, while T. lijiangense exhibited the best growth on YMTA medium. Mastering isolation and culturing skills of Tuber spp. to obtain mycelium culture can assist investigations into mutualistic relationships between Tuber spp. and different host trees, and also be applied to truffle production in the future.

並列關鍵字

Tuber ； isolation ； molecular identification ； microscopic features ； medium selection