Guided tissue regeneration (GTR) with non-absorbable membrane or absorbable membrane is a well-established treatment modality for periodontal disease. Platelet-rich plasma (PRP) is recently used to facilitate wound healing and hemostasis. The α granule of platelet contains coagulation factors. In addition, some growth factors released from α granule and PRP are considered as bone regeneration material. This article discusses the nature and characteristics of PRP, the processing of the materials, as well as the outcomes of using PRP for bone regeneration in various clinical situations.